PMID- 11101284
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190915
IS  - 1031-3613 (Print)
IS  - 1031-3613 (Linking)
VI  - 11
IP  - 7-8
DP  - 1999
TI  - Expression of bcl-2 and 3-beta hydroxysteroid dehydrogenase protein during oocyte 
      and follicle development in fetal and post-natal pig ovaries.
PG  - 463-70
AB  - The fetal and post-natal development of the pig ovary involves both proliferation 
      and apoptotic loss of germ cells, follicle formation and growth, and the 
      initiation of oocyte meiotic maturation. The present study measured the 
      expression of the proto-oncogene Bcl-2 immunohistochemically on paraffin sections 
      of pig ovaries to determine its relationship with folliculogenesis on Days 50 and 
      80 post coitum (p.c.) and on Days 1, 21, and 56 post partum (p.p.). The 
      expression of the steroidogenic enzyme 3beta-hydroxysteroid dehydrogenase 
      (3betaHSD) was used to determine the lineages of the cells forming the ovarian 
      follicles, and the expression of the cell proliferation-associated nuclear 
      antigen Ki-67 was used to determine germ cell proliferation and the initiation of 
      follicle growth. Expression of Ki-67 showed that many oogonia were proliferating 
      on Days 50 and 80 p.c. Granulosa cells were more proliferative on Day 56 p.p. 
      than at any other stage; Ki-67 was expressed in 70% of growing follicles and 
      granulosa cells had a 3% mean staining index per section. Less than 4% of germ 
      cells and follicles had morphological signs of degeneration during the period of 
      the study. Bcl-2 was most abundant on Days 21 p.p. and 56 p.p.; staining was 
      localized to stromal cells among follicles and in small clusters in the cortical 
      medullary junction (CMJ). 3BetaHSD staining on Day 50 p.c. was seen in cords of 
      stromal cells within the medulla of the ovary, and in the stromal cells investing 
      the oogonial nests. On Days 80 p.c., 1 p.p., 21 p.p., and 56 p.p., 3betaHSD was 
      expressed in the granulosa cells of primary or primordial follicles at the CMJ. 
      Production of Bcl-2 by somatic cells may support germ cell and preantral follicle 
      survival.
FAU - Garrett, W M
AU  - Garrett WM
AD  - Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, 
      Beltsville, MD 20705, USA.
FAU - Guthrie, H D
AU  - Guthrie HD
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*genetics
MH  - Animals
MH  - Apoptosis
MH  - Cell Nucleus/ultrastructure
MH  - Cytoplasm/ultrastructure
MH  - Female
MH  - Gene Expression
MH  - Ki-67 Antigen/analysis
MH  - Oocytes/*physiology/ultrastructure
MH  - Ovarian Follicle/*growth & development
MH  - Ovary/embryology/*growth & development/metabolism
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
MH  - Stromal Cells
MH  - *Swine
EDAT- 2000/12/02 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/02 11:00
PHST- 2000/12/02 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/02 11:00 [entrez]
AID - 10.1071/rd00003 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 1999;11(7-8):463-70. doi: 10.1071/rd00003.