PMID- 11101812
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20231213
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 18
IP  - 12
DP  - 2000 Dec
TI  - Tobacco ribosomal DNA spacer element stimulates amplification and expression of 
      heterologous genes.
PG  - 1303-6
AB  - Here we show that the cis-acting genetic element aps (amplification-promoting 
      sequence), isolated from the nontranscribed spacer region of tobacco ribosomal 
      DNA (rDNA), increases the level of expression of recombinant proteins. Transgenic 
      tobacco plants, transformed with expression cassettes containing the 
      herbicide-resistant acetolactate synthase (hr-ALS) gene or the green fluorescent 
      protein (GFP) gene fused to the aps sequence, had greater levels of corresponding 
      messenger RNAs (mRNAs) and proteins compared to transformants lacking aps. 
      Analysis of transgenic plants showed that aps increased the copy number and 
      transcription of the adjacent heterologous genes and, in the case of hr-ALS, 
      enhanced the herbicide resistance phenotype. Both the increased transgene copy 
      number and enhanced expression were stably inherited. These data provide the 
      first evidence that the aps sequence can be used for gene amplification in 
      transgenic plants and possibly other multicellular organisms.
FAU - Borisjuk, N
AU  - Borisjuk N
AD  - Biotech Center, Foran Hall, Cook College, Rutgers University, 59 Dudley Rd., New 
      Brunswick, NJ 08901-8520, USA.
FAU - Borisjuk, L
AU  - Borisjuk L
FAU - Komarnytsky, S
AU  - Komarnytsky S
FAU - Timeva, S
AU  - Timeva S
FAU - Hemleben, V
AU  - Hemleben V
FAU - Gleba, Y
AU  - Gleba Y
FAU - Raskin, I
AU  - Raskin I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (Herbicides)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.2.1.6 (Acetolactate Synthase)
SB  - IM
MH  - Acetolactate Synthase/genetics/metabolism
MH  - Base Sequence
MH  - DNA, Ribosomal Spacer/*genetics
MH  - Drug Resistance
MH  - *Gene Amplification
MH  - *Gene Expression
MH  - Herbicides/pharmacology
MH  - Molecular Sequence Data
MH  - Plants, Genetically Modified
MH  - *Plants, Toxic
MH  - Recombinant Proteins/*biosynthesis/genetics
MH  - Nicotiana/*genetics
MH  - Transcription, Genetic
MH  - *Transgenes
EDAT- 2000/12/02 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/02 11:00
PHST- 2000/12/02 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/02 11:00 [entrez]
AID - 10.1038/82430 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2000 Dec;18(12):1303-6. doi: 10.1038/82430.