PMID- 11102458
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20231104
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 20
IP  - 23
DP  - 2000 Dec 1
TI  - Neurobeachin: A protein kinase A-anchoring, beige/Chediak-higashi protein homolog 
      implicated in neuronal membrane traffic.
PG  - 8551-65
AB  - We describe the identification and initial characterization of neurobeachin, a 
      neuron-specific multidomain protein of 327 kDa with a high-affinity binding site 
      (K(d), 10 nm) for the type II regulatory subunit of protein kinase A (PKA RII). 
      Neurobeachin is peripherally associated with pleomorphic tubulovesicular 
      endomembranes near the trans sides of Golgi stacks and throughout the cell body 
      and cell processes. It is also found in a subpopulation of synapses, where it is 
      concentrated at the postsynaptic plasma membrane. In live cells, perinuclear 
      neurobeachin is dispersed by brefeldin A (BFA) within 1 min, and in permeabilized 
      cells a recruitment of neurobeachin from cytosol to Golgi-near membranes is 
      stimulated by GTPgammaS and prevented by brefeldin A. Spots of neurobeachin 
      recruitment are close to but distinct from recruitment sites of COP-I, AP-1, and 
      AP-3 coat proteins involved in vesicle budding. These observations indicate that 
      neurobeachin binding to membranes close to the trans-Golgi requires an 
      ADP-ribosylation factor-like GTPase, possibly in association with a novel type of 
      protein coat. A neurobeachin isoform that does not bind RII, beige-like protein 
      (BGL), is expressed in many tissues. Neurobeachin, BGL, and approximately 10 
      other mammalian gene products share a characteristic C-terminal BEACH-WD40 
      sequence module, which is also present in gene products of invertebrates, plants, 
      protozoans, and yeasts, thus defining a new protein family. The prototype member 
      of this family of BEACH domain proteins, lysosomal trafficking regulator (LYST), 
      is deficient in genetic defects of protein sorting in lysosome biogenesis (the 
      beige mouse and Chediak-Higashi syndrome). Neurobeachin's subcellular 
      localization, its coat protein-like membrane recruitment, and its sequence 
      similarity to LYST suggest an involvement in neuronal post-Golgi membrane 
      traffic, one of its functions being to recruit protein kinase A to the membranes 
      with which it associates.
FAU - Wang, X
AU  - Wang X
AD  - Institut fur Physiologische Chemie and Institut fur Anatomie, Ruhr-Universitat 
      Bochum, D-44780 Bochum, Germany.
FAU - Herberg, F W
AU  - Herberg FW
FAU - Laue, M M
AU  - Laue MM
FAU - Wullner, C
AU  - Wullner C
FAU - Hu, B
AU  - Hu B
FAU - Petrasch-Parwez, E
AU  - Petrasch-Parwez E
FAU - Kilimann, M W
AU  - Kilimann MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Carrier Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Lyst protein, mouse)
RN  - 0 (Lyst protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nbea protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (che-2 protein, C elegans)
RN  - 20350-15-6 (Brefeldin A)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Type II)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Binding Sites/genetics
MH  - Brefeldin A/pharmacology
MH  - COP-Coated Vesicles/metabolism
MH  - COS Cells
MH  - Carrier Proteins/*genetics/metabolism
MH  - Chediak-Higashi Syndrome/*genetics
MH  - Chickens
MH  - Cloning, Molecular
MH  - Cyclic AMP-Dependent Protein Kinase Type II
MH  - Cyclic AMP-Dependent Protein Kinases/*metabolism
MH  - Cytosol/metabolism
MH  - Golgi Apparatus/drug effects/metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Helminth Proteins/genetics
MH  - Intracellular Membranes/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Membrane Proteins
MH  - Mice
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Neurons/cytology/drug effects/*metabolism
MH  - PC12 Cells
MH  - Protein Structure, Tertiary/genetics
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Protein Transport/drug effects/genetics
MH  - Proteins/*genetics
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Vesicular Transport Proteins
PMC - PMC6773050
EDAT- 2000/01/11 19:15
MHDA- 2001/03/03 10:01
PMCR- 2001/06/01
CRDT- 2000/01/11 19:15
PHST- 2000/01/11 19:15 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/01/11 19:15 [entrez]
PHST- 2001/06/01 00:00 [pmc-release]
AID - 20/23/8551 [pii]
AID - 4693 [pii]
AID - 10.1523/JNEUROSCI.20-23-08551.2000 [doi]
PST - ppublish
SO  - J Neurosci. 2000 Dec 1;20(23):8551-65. doi: 10.1523/JNEUROSCI.20-23-08551.2000.