PMID- 11102572
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 885
IP  - 2
DP  - 2000 Dec 8
TI  - Corticosterone inhibits generation of long-term potentiation in rat hippocampal 
      slice: involvement of brain-derived neurotrophic factor.
PG  - 182-91
AB  - In the present study, the effect of corticosterone (CORT) on the generation of 
      long-term potentiation (LTP) and its underlying mechanism involving neurotrophin 
      gene expression in CA1 synapses of rat hippocampal slice were examined. Our 
      experimental results showed incubation of hippocampal slice with CORT for 3 h had 
      no effect on either the slope or amplitude of excitatory postsynaptic potentials 
      (EPSP) evoked in hippocampal CA1 pyramidal dentrites, indicating no marked change 
      in basal synaptic transmission. However, when tetanic stimulation (100 pulses, 
      100 Hz) was delivered to the Schaffer collateral pathway, CORT application 
      significantly attenuated the tetanus-induced increases of both EPSP slope and 
      amplitude, demonstrating an inhibitory effect of CORT on LTP generation. In 
      addition, CORT treatment significantly reduced both slope and amplitude ratios of 
      the second evoked EPSP to the first one when paired-pulse facilitation (PPF) was 
      established at different interpulse intervals from 20 to 40 ms, suggesting that a 
      presynaptic mechanism may be involved in CORT-induced hippocampal synaptic 
      plasticity. Reverse-transcription polymerase chain reaction (RT-PCR) analysis 
      showed that CORT-treated hippocampal CA1 cells underwent a significant decrease 
      in the expression of mRNA for nerve growth factor-beta (NGF-beta) and 
      brain-derived neurotrophic factor (BDNF), but not for neurotrophin-3 (NT-3) 
      compared with those in control. Moreover, BDNF co-applied with CORT significantly 
      antagonized CORT-induced deficit in PPF. Taken together, the present results 
      suggest that CORT-induced inhibition of LTP may be, at least to some extent, 
      mediated by a presynaptic mechanism and decrease in the BDNF expression in rat 
      hippocampal CA1 cells induced by CORT may partially account for this presynaptic 
      mechanism.
FAU - Zhou, J
AU  - Zhou J
AD  - Laboratory of Neuropharmacology, Beijing Institute of Pharmacology and 
      Toxicology, Beijing 100850, PR China. jzhou@codon.nih.gov
FAU - Zhang, F
AU  - Zhang F
FAU - Zhang, Y
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/*drug effects/metabolism/pharmacology
MH  - Corticosterone/*pharmacology
MH  - Excitatory Postsynaptic Potentials/*drug effects/physiology
MH  - Gene Expression/drug effects/physiology
MH  - Hippocampus/*drug effects/physiology
MH  - Long-Term Potentiation/*drug effects/physiology
MH  - Male
MH  - Nerve Growth Factor
MH  - Neurotrophin 3/drug effects/metabolism
MH  - RNA, Messenger/drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2000/12/05 11:00
MHDA- 2001/03/07 10:01
CRDT- 2000/12/05 11:00
PHST- 2000/12/05 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2000/12/05 11:00 [entrez]
AID - S0006-8993(00)02934-6 [pii]
AID - 10.1016/s0006-8993(00)02934-6 [doi]
PST - ppublish
SO  - Brain Res. 2000 Dec 8;885(2):182-91. doi: 10.1016/s0006-8993(00)02934-6.