PMID- 11102683
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20190915
IS  - 0168-3659 (Print)
IS  - 0168-3659 (Linking)
VI  - 69
IP  - 3
DP  - 2000 Dec 3
TI  - Protein encapsulation into biodegradable microspheres by a novel S/O/W emulsion 
      method using poly(ethylene glycol) as a protein micronization adjuvant.
PG  - 435-44
AB  - A new method for preparing protein-loaded biodegradable microspheres by a process 
      involving solid-in-oil-in-water (S/O/W) emulsion was established using 
      poly(ethylene glycol) (PEG). In the first step, a protein solution was 
      lyophilized with PEG, which resulted in the formation of spherical protein 
      microparticles, less than 5 microm in diameter, dispersed in a continuous PEG 
      phase. This process was well explained by the aqueous phase separation phenomenon 
      induced by freezing-condensation. Since this lyophilizate could be directly 
      dispersed in an organic phase containing biodegradable polymer by dissolving PEG 
      with methylene chloride, a conventional in-water drying method could be adopted 
      in the second step. Through this S/O/W emulsion process, horseradish peroxidase 
      was effectively entrapped into monolithic-type microspheres of 
      poly(DL-lactic-co-glycolic acid) (PLGA), without significant loss of activity. 
      Bovine superoxide dismutase (bSOD), as another model protein, could be 
      encapsulated into reservoir-type microspheres by the 'polymer-alloys method' 
      using both poly(DL-lactic acid) (PLA) and PLGA. The initial release of bSOD from 
      this reservoir-type microsphere was efficiently reduced. Further, the bSOD 
      release kinetics could be suitably modified by adjusting the loading amounts of 
      PEG or polymer composition. In this study, the multi-functional nature of PEG was 
      successfully utilized in the preparation and designing of protein-loaded 
      microspheres.
FAU - Morita, T
AU  - Morita T
AD  - DDS Research Department, Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd., 
      3-16-89 Kashima, Yodogawa-ku, 532-8505, Osaka, Japan. t-morita@tanabe.com
FAU - Sakamura, Y
AU  - Sakamura Y
FAU - Horikiri, Y
AU  - Horikiri Y
FAU - Suzuki, T
AU  - Suzuki T
FAU - Yoshino, H
AU  - Yoshino H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Emulsions)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Emulsions
MH  - Lactic Acid/administration & dosage
MH  - Microspheres
MH  - Polyethylene Glycols
MH  - Polyglycolic Acid/administration & dosage
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/administration & dosage
MH  - Proteins/*administration & dosage
MH  - Superoxide Dismutase/administration & dosage
MH  - *Technology, Pharmaceutical
EDAT- 2000/12/05 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/05 11:00
PHST- 2000/12/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/05 11:00 [entrez]
AID - S0168365900003266 [pii]
AID - 10.1016/s0168-3659(00)00326-6 [doi]
PST - ppublish
SO  - J Control Release. 2000 Dec 3;69(3):435-44. doi: 10.1016/s0168-3659(00)00326-6.