PMID- 11104025
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 122
IP  - 1
DP  - 2000 Oct 1
TI  - Comparison of comparative genomic hybridization and interphase fluorescence in 
      situ hybridization in ovarian carcinomas: possibilities and limitations of both 
      techniques.
PG  - 7-12
AB  - Comparative genomic hybridization (CGH) is a valuable technique for cytogenetic 
      analysis of solid tumors. To evaluate the reliability of CGH, we examined DNA of 
      10 ovarian carcinomas after CGH analysis with single- and double-locus 
      fluorescence in situ hybridization (FISH). The FISH experiments, involving 5 
      chromosomes (chromosomes 3, 6, 8, 12, and 18) with different FISH probes, 
      confirmed the CGH results in 66.2% of cases (92 of 139 investigated loci). In 4 
      patients, inconsistent results (41 loci) were related to polyploidy, because CGH 
      cannot detect polyploid karyotypes. The remaining 6 discordant loci can be 
      referred to limitations in both techniques. Re-evaluation of FISH and CGH results 
      by one other is therefore recommended to overcome these technical artifacts. 
      Nevertheless, CGH is of potential value in characterizing chromosomal alterations 
      and might help in generating tumor-specific sets of FISH probes to obtain genetic 
      information of prognostic value within a few days.
FAU - Jacobsen, A
AU  - Jacobsen A
AD  - Department of Gynecology and Obstetrics, University of Kiel, Germany.
FAU - Arnold, N
AU  - Arnold N
FAU - Weimer, J
AU  - Weimer J
FAU - Kiechle, M
AU  - Kiechle M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Chromosomes, Human
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - *Nucleic Acid Hybridization
MH  - Ovarian Neoplasms/*genetics/pathology
MH  - Reproducibility of Results
EDAT- 2000/12/05 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/05 11:00
PHST- 2000/12/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/05 11:00 [entrez]
AID - S0165-4608(00)00263-6 [pii]
AID - 10.1016/s0165-4608(00)00263-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Oct 1;122(1):7-12. doi: 
      10.1016/s0165-4608(00)00263-6.