PMID- 11104504
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20031114
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 62
IP  - 5
DP  - 2000 Dec 1
TI  - Increase in neurotrophin-3 expression followed by Purkinje cell degeneration in 
      the adult rat cerebellum after spinal cord transection.
PG  - 668-74
AB  - Changes in brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) 
      contents following thoracic spinal cord transection were investigated in the 
      cerebral cortex, hippocampus, and cerebellum of rats. The NT-3 content became 
      significantly elevated at 3 days after transection only in the cerebellum and 
      gradually declined to the control level by 6 days after the injury, remaining 
      unchanged in the cerebral cortex and hippocampus. No significant change in the 
      BDNF content was observed in any of the regions tested. Immunohistochemical 
      analysis showed that the labeling indicating NT-3-like immunoreactivity was 
      intensified in both cerebellar granule and Purkinje cells 3 days after the 
      injury. The number of Purkinje cells with aggregation of chromatin around the 
      nuclear membrane and swelling of the cytoplasm and/or organelles gradually 
      increased with time starting 4 days after the injury, demonstrating morphological 
      changes indicative of necrosis. However, no abnormal morphology was found in 
      cerebellar granule cells at any time examined. We suggest that it is reasonable 
      that increased NT-3 stimulated the death of Purkinje cells, because 1) the 
      degeneration was necrosis, which is known to be accelerated by neurotrophins 
      under certain pathological conditions, and 2) the increase in NT-3 occurred prior 
      to Purkinje cell degeneration. Therefore, our present results may imply that 
      spinal cord injury-induced NT-3 accelerates injury rather than alleviates 
      degeneration of Purkinje cells.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Kawakami, H
AU  - Kawakami H
AD  - Department of Orthopedic Surgery, Nagoya University, Showa-ku, Nagoya, Aichi, 
      Japan. hirosi-k@tsuru.med.nagoya-u.ac.jp
FAU - Nitta, A
AU  - Nitta A
FAU - Matsuyama, Y
AU  - Matsuyama Y
FAU - Kamiya, M
AU  - Kamiya M
FAU - Satake, K
AU  - Satake K
FAU - Sato, K
AU  - Sato K
FAU - Kondou, K
AU  - Kondou K
FAU - Iwata, H
AU  - Iwata H
FAU - Furukawa, S
AU  - Furukawa S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
SB  - IM
MH  - Animals
MH  - Brain/metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cerebellum/*metabolism/pathology
MH  - Female
MH  - Immunohistochemistry
MH  - Microscopy, Electron
MH  - Necrosis
MH  - Neurotrophin 3/*metabolism
MH  - Purkinje Cells/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*pathology
MH  - Spinal Cord Injuries/metabolism/pathology
MH  - Up-Regulation
EDAT- 2000/12/05 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/05 11:00
PHST- 2000/12/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/05 11:00 [entrez]
AID - 10.1002/1097-4547(20001201)62:5<668::AID-JNR5>3.0.CO;2-N [pii]
AID - 10.1002/1097-4547(20001201)62:5<668::AID-JNR5>3.0.CO;2-N [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 Dec 1;62(5):668-74. doi: 
      10.1002/1097-4547(20001201)62:5<668::AID-JNR5>3.0.CO;2-N.