PMID- 11104508
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20121115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 62
IP  - 5
DP  - 2000 Dec 1
TI  - Insulin-like growth factor-I (IGF-I) protects myelination from undernutritional 
      insult: studies of transgenic mice overexpressing IGF-I in brain.
PG  - 700-8
AB  - Using insulin-like growth factor-I (IGF-I)-overexpressing transgenic (Tg) mice as 
      a model, we have shown that IGF-I promotes myelination by increasing the number 
      of oligodendrocytes and stimulating the expression of myelin-specific protein 
      genes. In the present study, we investigated whether IGF-I protects myelination 
      from undernutritional insult in Tg mice. Mice were undernourished beginning from 
      postnatal (P) day 1, a time coincident with the onset of transgene expression, 
      and sacrificed at P20. Consistently with our previous studies, brain weights of 
      undernourished non-Tg control mice were decreased by approximately 18%. Brain 
      weights of undernourished IGF-I Tg mice, however, were the same as those of 
      well-fed control mice and much greater than those of undernourished control mice. 
      The expression of two major myelin proteins [myelin basic protein (MBP) and 
      proteolipid protein (PLP)] in cerebral cortex (CTX) and hippocampus (HIP) was 
      decreased by 73-92% in undernourished control mice, as judged by Northern and 
      Western blot hybridization. The abundances of MBP and PLP mRNAs and proteins, 
      however, were decreased by only 40-70% in undernourished IGF-I Tg mice. To assess 
      the number of oligodendrocytes and their precursors, antibodies specific for 
      carbonic anhydrase II (CAII; an oligodendrocyte marker) and NG2 (a precursor 
      marker) were used. Compared to their well-fed counterparts, undernourished 
      control mice exhibited 17-30% decreases in the number of oligodendrocytes and 
      their precursors in CTX and corpus callosum (CC), whereas well-fed IGF-I Tg mice 
      had 21-35% increases in CTX and CC. Undernourished IGF-I Tg mice exhibited cell 
      numbers similar to those of well-fed control mice. These data indicate that IGF-I 
      protects myelination from undernutrition damage during development.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Ye, P
AU  - Ye P
AD  - Department of Pediatrics, The University of North Carolina at Chapel Hill, Chapel 
      Hill, North Carolina 27599-7220, USA. Ping_Ye@med.unc.edu
FAU - Lee, K H
AU  - Lee KH
FAU - D'Ercole, A J
AU  - D'Ercole AJ
LA  - eng
GR  - HD08299/HD/NICHD NIH HHS/United States
GR  - NS38891/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Myelin Proteins)
RN  - 0 (Myelin Proteolipid Protein)
RN  - 0 (RNA, Messenger)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Brain/growth & development/*metabolism
MH  - Cell Count
MH  - Cerebral Cortex/cytology/growth & development
MH  - Corpus Callosum/cytology/growth & development
MH  - *Food Deprivation
MH  - Immunohistochemistry
MH  - Insulin-Like Growth Factor I/genetics/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Myelin Basic Protein/metabolism
MH  - Myelin Proteins/*metabolism
MH  - Myelin Proteolipid Protein/metabolism
MH  - Oligodendroglia/cytology
MH  - RNA, Messenger/metabolism
EDAT- 2000/12/05 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/05 11:00
PHST- 2000/12/05 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/05 11:00 [entrez]
AID - 10.1002/1097-4547(20001201)62:5<700::AID-JNR9>3.0.CO;2-1 [pii]
AID - 10.1002/1097-4547(20001201)62:5<700::AID-JNR9>3.0.CO;2-1 [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 Dec 1;62(5):700-8. doi: 
      10.1002/1097-4547(20001201)62:5<700::AID-JNR9>3.0.CO;2-1.