PMID- 11105760
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20231213
IS  - 1355-8382 (Print)
IS  - 1469-9001 (Electronic)
IS  - 1355-8382 (Linking)
VI  - 6
IP  - 11
DP  - 2000 Nov
TI  - RBP45 and RBP47, two oligouridylate-specific hnRNP-like proteins interacting with 
      poly(A)+ RNA in nuclei of plant cells.
PG  - 1610-24
AB  - Introns in plant nuclear pre-mRNAs are highly enriched in U or U + A residues and 
      this property is essential for efficient splicing. Moreover, 3'-untranslated 
      regions (3'-UTRs) in plant pre-mRNAs are generally UA-rich and contain sequences 
      that are important for the polyadenylation reaction. Here, we characterize two 
      structurally related RNA-binding proteins (RBPs) from Nicotiana plumbaginifolia, 
      referred to as RBP45 and RBP47, having specificity for oligouridylates. Both 
      proteins contain three RBD-type RNA-binding domains and a glutamine-rich 
      N-terminus, and share similarity with Nam8p, a protein associated with U1 snRNP 
      in the yeast Saccharomyces cerevisiae. Deletion analysis of RBP45 and RBP47 
      indicated that the presence of at least two RBD are required for interaction with 
      RNA and that domains other than RBD do not significantly contribute to binding. 
      mRNAs for RBP45 and RBP47 and mRNAs encoding six related proteins in Arabidopsis 
      thaliana are constitutively expressed in different plant organs. Indirect 
      immunofluorescence and fractionation of cell extracts showed that RBP45 and RBP47 
      are localized in the nucleus. In vivo UV crosslinking experiments demonstrated 
      their association with the nuclear poly(A)+ RNA. In contrast to UBP1, another 
      oligouridylate-binding nuclear three-RBD protein of N. plumbaginifolia (Lambermon 
      et al., EMBO J, 2000, 19:1638-1649), RBP45 and RBP47 do not stimulate mRNA 
      splicing and accumulation when transiently overexpressed in protoplasts. 
      Properties of RBP45 and RBP47 suggest they represent hnRNP-proteins participating 
      in still undefined steps of pre-mRNA maturation in plant cell nuclei.
FAU - Lorkovic, Z J
AU  - Lorkovic ZJ
AD  - Friedrich Miescher-Institut, Basel, Switzerland.
FAU - Wieczorek Kirk, D A
AU  - Wieczorek Kirk DA
FAU - Klahre, U
AU  - Klahre U
FAU - Hemmings-Mieszczak, M
AU  - Hemmings-Mieszczak M
FAU - Filipowicz, W
AU  - Filipowicz W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Oligoribonucleotides)
RN  - 0 (Plant Proteins)
RN  - 0 (RBP45 protein, Nicotiana plumbaginifolia)
RN  - 0 (RBP47 protein, Nicotiana plumbaginifolia)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Uracil Nucleotides)
RN  - 0 (oligo(U))
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/*genetics/metabolism
MH  - Binding Sites
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - Escherichia coli
MH  - Evolution, Molecular
MH  - Molecular Sequence Data
MH  - Oligoribonucleotides/*metabolism
MH  - Phylogeny
MH  - *Plant Proteins
MH  - *Plants, Toxic
MH  - RNA, Messenger/*metabolism
MH  - RNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Nicotiana/*genetics/metabolism
MH  - Uracil Nucleotides/*metabolism
PMC - PMC1370030
EDAT- 2000/12/06 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/11/01
CRDT- 2000/12/06 11:00
PHST- 2000/12/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/06 11:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - 10.1017/s1355838200001163 [doi]
PST - ppublish
SO  - RNA. 2000 Nov;6(11):1610-24. doi: 10.1017/s1355838200001163.