PMID- 11106329
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 46
IP  - 12
DP  - 2000 Dec
TI  - Determination of the designer drugs 3, 4-methylenedioxymethamphetamine, 
      3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC 
      and fluorescence detection in whole blood, serum, vitreous humor, and urine.
PG  - 1968-77
AB  - BACKGROUND: The popular designer drugs 3, 4-methylenedioxymethamphetamine (MDMA) 
      and 3, 4-methylenedioxyethylamphetamine (MDEA) can be determined in serum, whole 
      blood, and urine, but also in vitreous humor. The latter matrix is interesting 
      when dealing with decomposed bodies in a toxicological setting. METHODS: After 
      extraction, chromatographic separation was achieved on a narrow-bore C(18) column 
      by gradient elution with fluorometric detection; results were confirmed by liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: The method was 
      linear over the range of 2-1000 microg/L for whole blood, serum, and vitreous 
      humor, and 0.1-5 mg/L for urine. Extraction recoveries were >70%, imprecision 
      (CV) was 2.5-19%, and analytical recoveries were 95.5-104.4%. The limit of 
      detection (LOD) and the limit of quantification (LOQ) were 0.8 and 2 microg/L, 
      respectively, for whole blood, serum, and vitreous humor, and 2.5 microg/L and 
      0.1 mg/L, respectively, for urine. Excellent correlations between the 
      quantitative LC-fluorescence and LC-MS/MS results were obtained. We found the 
      following concentrations in a thanatochemical distribution study in rabbits: in 
      serum, 5.3-685 microg/L for MDMA and from the LOQ to 14.5 microg/L for 3, 
      4-methylenedioxyamphetamine (MDA); in whole blood, 19.7-710 microg/L for MDMA and 
      from the LOQ to 17.8 microg/L for MDA; in vitreous humor, 12.1-97.8 microg/L for 
      MDMA and from the LOQ to 3.86 microg/L for MDA. In routine toxicological urine 
      samples, concentrations ranged from LOQ to 14.62 mg/L for MDA, from LOQ to 157 
      mg/L for MDMA, and from LOQ to 32.54 mg/L for MDEA. CONCLUSIONS: The HPLC method 
      described is sensitive, specific, and suitable for the determination of MDMA, 
      MDEA, and MDA in whole blood, serum, vitreous humor, and urine.
FAU - Clauwaert, K M
AU  - Clauwaert KM
AD  - Laboratory of Toxicology and Laboratory of Medicinal Chemistry, Ghent University, 
      Harelbekestraat 72, B-9000 Gent, Belgium.
FAU - Van Bocxlaer, J F
AU  - Van Bocxlaer JF
FAU - De Letter, E A
AU  - De Letter EA
FAU - Van Calenbergh, S
AU  - Van Calenbergh S
FAU - Lambert, W E
AU  - Lambert WE
FAU - De Leenheer, A P
AU  - De Leenheer AP
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Designer Drugs)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - ML1I4KK67B (3,4-methylenedioxyethamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analogs & derivatives/*analysis/blood/urine
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Designer Drugs/*analysis
MH  - Humans
MH  - Mass Spectrometry
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis/blood/urine
MH  - Rabbits
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence
MH  - Substance Abuse Detection/*methods
MH  - Vitreous Body/*chemistry
EDAT- 2000/12/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/06 11:00
PHST- 2000/12/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/06 11:00 [entrez]
PST - ppublish
SO  - Clin Chem. 2000 Dec;46(12):1968-77.