PMID- 11106817
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 122
IP  - 2
DP  - 2000 Oct 15
TI  - Molecular and cytogenetic analysis of glioblastoma multiforme.
PG  - 87-92
AB  - Glioblastoma multiforme (GBM) is the most common primary tumor occurring in the 
      central nervous system of adults. Although progress has been made in clinical 
      management of this tumor, little is known about the molecular defects underlying 
      the initiation and progression of GBM. To address these issues, we have 
      characterized five cases of GBM using cytogenetics, comparative genomic 
      hybridization (CGH), fluorescence in situ hybridization (FISH), and direct 
      sequencing. All of these tumors were observed to have clonal chromosome 
      aberrations. Complicated chromosome translocations including der(18)t(2;4;12;18), 
      der(X)t(X;10)(q27.1;p12.1) and der(10)t(10;15)(p11.23;q11.2), and der(1) 
      (:1p31-->1q44::7q11. 3-->7qter) were seen in three tumors. Loss of the CDKN2 gene 
      was noted in four tumors. A gain of copy number of the Cathepsin L gene was seen 
      in two tumors. Amplification of the CDK4, MDM2, and GLI/CHOP genes was noted in 
      two tumors, and amplification of the PDGFR gene was detected in one tumor. 
      Mutation of exon 5 of the TP53 gene was found in three tumors. No mutation of the 
      BCL10 gene was detected in five cases of GBM analyzed, although deletion of 
      chromosome 1p was seen in two tumors. These results provide information for 
      further investigation of GBM.
FAU - Mao, X
AU  - Mao X
AD  - Human Cytogenetics Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn 
      Fields, WC2A 3PX, London, UK. mxmayo@hotmail.com
FAU - Hamoudi, R A
AU  - Hamoudi RA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Cytogenetic Analysis
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Gene Amplification
MH  - Gene Deletion
MH  - Glioblastoma/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Nucleic Acid Hybridization
MH  - Translocation, Genetic
EDAT- 2000/12/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/07 11:00
PHST- 2000/12/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/07 11:00 [entrez]
AID - S0165-4608(00)00278-8 [pii]
AID - 10.1016/s0165-4608(00)00278-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Oct 15;122(2):87-92. doi: 
      10.1016/s0165-4608(00)00278-8.