PMID- 11107171
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 30
IP  - 1
DP  - 2001 Jan
TI  - Cross-species color banding in ten cases of myeloid malignancies with complex 
      karyotypes.
PG  - 15-24
AB  - Cross-species color banding is a multiple-color fluorescence in situ 
      hybridization (FISH) technique using probes developed from other animal species. 
      Hybridization to human metaphases produces color banding patterns specific for 
      each homologous chromosome pair. The technique has been evaluated in a 
      complementary manner with G-banding and chromosome painting in a series of 10 
      myeloid malignancies with complex or unresolved karyotypes. Color banding 
      detected the majority of chromosomal abnormalities, which had been identified by 
      G-banding and in each case revealed chromosomal changes that G-banding had not 
      identified. Painting was necessary to confirm these abnormalities due to the 
      limitation of only seven colors in the color-banded karyotype. At the same time, 
      painting fortuitously uncovered cryptic abnormalities in 6 of 10 cases that had 
      not been detected by color banding. Insertions were visible by painting only. 
      This study has demonstrated that in the analysis of complex karyotypes, the 
      application of color banding revealed the involvement of the long arm of 
      chromosome 3, indicating a poor risk, in two cases not identified by G-banding. 
      Therefore, these techniques applied together have revealed cryptic chromosomal 
      abnormalities with prognostic significance, which in some cases may have 
      implications for patient management.
FAU - Harrison, C J
AU  - Harrison CJ
AD  - Department of Haematology, Royal Free and University College Medical School, 
      London, United Kingdom. cjh@rfc.ucl.ac.uk
FAU - Yang, F
AU  - Yang F
FAU - Butler, T
AU  - Butler T
FAU - Cheung, K L
AU  - Cheung KL
FAU - O'Brien, P C
AU  - O'Brien PC
FAU - Hennessy, B J
AU  - Hennessy BJ
FAU - Prentice, H G
AU  - Prentice HG
FAU - Ferguson-Smith, M
AU  - Ferguson-Smith M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Coloring Agents)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Chromosome Aberrations/genetics/pathology
MH  - Chromosome Banding/*methods
MH  - Chromosome Disorders
MH  - Coloring Agents
MH  - Female
MH  - Humans
MH  - Hylobates
MH  - Karyotyping
MH  - Leukemia, Erythroblastic, Acute/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
MH  - Species Specificity
EDAT- 2000/12/07 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/07 11:00
PHST- 2000/12/07 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/07 11:00 [entrez]
AID - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1061>3.0.CO;2-U [pii]
AID - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1061>3.0.co;2-u [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Jan;30(1):15-24. doi: 
      10.1002/1098-2264(2000)9999:9999<::aid-gcc1061>3.0.co;2-u.