PMID- 11107184
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 30
IP  - 1
DP  - 2001 Jan
TI  - Chromosome arm-specific multicolor FISH.
PG  - 105-9
AB  - Several systems for 24-color fluorescence in situ hybridization (FISH) have been 
      developed and applied to karyotyping and detection of chromosomal abnormalities. 
      We have developed a 42-color multicolor FISH (mFISH) technique (armFISH), which 
      permits the detection of chromosomal aberrations at the resolution of chromosome 
      arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, 
      MetaSystems GmbH) supplemented with a set of differentially labeled chromosome 
      arm-specific painting probes (arm-kit, comprising either p- or q-arms of all 
      human chromosomes, except the p-arm of the acrocentric- and Y chromosomes). The 
      mFISH-probe cocktail and the arm-kit are combined and hybridized together to 
      metaphase chromosomes. The armFISH is analyzed in two steps; first, the 
      conventional mFISH image analysis is performed, followed by the arm-kit analysis 
      to reveal the chromosome arms involved. The examples demonstrate the utility of 
      armFISH in defining chromosomal rearrangements of human cancers.
FAU - Karhu, R
AU  - Karhu R
AD  - Laboratory of Cancer Genetics, University of Tampere and Tampere University 
      Hospital, Tampere, Finland. ritva.karhu@uta.fi
FAU - Ahlstedt-Soini, M
AU  - Ahlstedt-Soini M
FAU - Bittner, M
AU  - Bittner M
FAU - Meltzer, P
AU  - Meltzer P
FAU - Trent, J M
AU  - Trent JM
FAU - Isola, J J
AU  - Isola JJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 0 (cyanine dye 5)
SB  - IM
MH  - Carbocyanines
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Painting/*methods
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Reagent Kits, Diagnostic
MH  - Sensitivity and Specificity
EDAT- 2000/12/07 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/07 11:00
PHST- 2000/12/07 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/07 11:00 [entrez]
AID - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1068>3.0.CO;2-9 [pii]
AID - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1068>3.0.co;2-9 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Jan;30(1):105-9. doi: 
      10.1002/1098-2264(2000)9999:9999<::aid-gcc1068>3.0.co;2-9.