PMID- 11108465
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 12
IP  - 6
DP  - 2000 Nov
TI  - Comparison of virus isolation, reverse transcription-polymerase chain reaction, 
      immunohistochemistry, and in situ hybridization for the detection of porcine 
      reproductive and respiratory syndrome virus from naturally aborted fetuses and 
      stillborn piglets.
PG  - 582-7
AB  - Virus isolation, reverse transcription-polymerase chain reaction (RT-PCR), 
      immunohistochemistry, and in situ hybridization methods were compared for the 
      detection of porcine reproductive and respiratory syndrome virus (PRRSV). Seven 
      aborted fetuses and 6 stillborn piglets naturally infected with PRRSV were used 
      in the study. Viral antigen and viral nucleic acid were detected in macrophages 
      and dendritic cells in the spleen, tonsil, lymph nodes, and thymus; in 
      macrophages of liver, heart, and lung; and in endothelial cells and myocytes of 
      the heart. Viral antigen and viral nucleic acid were most consistently detected 
      in the spleen. Of the 13 samples, 6 were positive for PRRSV by all 4 techniques. 
      Four (31%) samples were positive for PRRSV by RT-PCR, in situ hybridization, and 
      virus isolation. Two (15%) samples were positive for PRRSV by virus isolation, 
      RT-PCR, and in situ hybridization. One (8%) was positive for PRRSV by virus 
      isolation and RT-PCR. The RT-PCR identified the presence of PRRSV more frequently 
      than the other methods. However, when only formalin-fixed tissues are submitted, 
      immunohistochemistry and in situ hybridization would be useful methods for the 
      detection of PRRSV antigen and nucleic acid.
FAU - Cheon, D S
AU  - Cheon DS
AD  - Department of Veterinary Pathology, College of Veterinary Medicine and School of 
      Agricultural Biotechnology, Seoul National University, Suwon, Kyounggi-Do, 
      Republic of Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Abortion, Veterinary/pathology/*virology
MH  - Animals
MH  - Female
MH  - Fetal Death/pathology/*veterinary/virology
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization/methods
MH  - Macrophages/pathology/virology
MH  - Polymerase Chain Reaction/methods
MH  - Porcine Reproductive and Respiratory Syndrome/embryology/*pathology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Pregnancy
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
EDAT- 2000/12/07 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/12/07 11:00
PHST- 2000/12/07 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/12/07 11:00 [entrez]
AID - 10.1177/104063870001200619 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2000 Nov;12(6):582-7. doi: 10.1177/104063870001200619.