PMID- 11109968
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20191104
IS  - 0951-3590 (Print)
IS  - 0951-3590 (Linking)
VI  - 14
IP  - 5
DP  - 2000 Oct
TI  - Effect of T-helper 1 cytokines on secretion of T-helper 2 cytokines by term 
      trophoblast cells in culture.
PG  - 305-10
AB  - A successful pregnancy has been postulated to be the result of a discrete balance 
      between T-helper 1 (Th1) and T-helper 2 (Th2) type cytokines involved in growth 
      and development of the conceptus. The aim of the present study was to examine the 
      effect of Th1 cytokines (interleukin-1 beta (IL-1 beta) and tumor necrosis 
      factor-alpha (TNF alpha)) on the release of Th2 cytokines including IL-6 and 
      IL-10 by trophoblast cells obtained from term placenta. Trophoblast cells 
      isolated by enzymatic disaggregation and Percoll gradient fractionation were 
      cultured in supplemented medium alone or with varying concentrations of the 
      selected recombinant cytokines. After 48 h of incubation, samples of the culture 
      supernatant were analyzed for the Th2 cytokines IL-6 and IL-10 using specific 
      ELISA assays. Both IL-1 beta and TNF alpha had no effect on the cell number and 
      viability as determined by MTT assay. IL-1 beta significantly stimulated 
      trophoblast release of IL-6 in a dose-dependent manner (3.3-, 5.5-, 10.3- and 
      22.4-fold higher compared to the control at 10, 50, 100, 500 U IL-1 beta/ml 
      respectively, p < 0.05). TNF alpha also stimulated release of IL-6 by these 
      cells. However, the stimulation at lower concentrations was not very high and a 
      significant (p < 0.05) stimulation was observed only at higher concentrations 
      (1.1-, 1.3-, 2.6- and 5.9-fold higher at 500, 1000, 1500, 2000 U TNF alpha/ml 
      respectively). In contrast, neither IL-1 beta or TNF alpha exerted any 
      significant effect on IL-10 release by term trophoblast cells (p > 0.05). The 
      results of this study provide evidence that production of Th2 cytokines might be 
      under the control of different regulatory pathways.
FAU - Agarwal, R
AU  - Agarwal R
AD  - Department of Obstetrics and Gynecology, National University Hospital, National 
      University of Singapore, Singapore.
FAU - Loganath, A
AU  - Loganath A
FAU - Roy, A C
AU  - Roy AC
FAU - Wong, Y C
AU  - Wong YC
FAU - Ng, S C
AU  - Ng SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gynecol Endocrinol
JT  - Gynecological endocrinology : the official journal of the International Society 
      of Gynecological Endocrinology
JID - 8807913
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Cells, Cultured
MH  - Cesarean Section
MH  - Coculture Techniques
MH  - Female
MH  - Humans
MH  - Interleukin-1/*pharmacology
MH  - Interleukin-10/analysis/*biosynthesis
MH  - Interleukin-6/analysis/*biosynthesis
MH  - Placenta/*cytology
MH  - Pregnancy
MH  - Recombinant Proteins/pharmacology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
MH  - Trophoblasts/*cytology/drug effects/immunology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2000/12/08 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/12/08 11:00
PHST- 2000/12/08 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/12/08 11:00 [entrez]
AID - 10.3109/09513590009167697 [doi]
PST - ppublish
SO  - Gynecol Endocrinol. 2000 Oct;14(5):305-10. doi: 10.3109/09513590009167697.