PMID- 11110683
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 13
DP  - 2000 Dec 15
TI  - Primitive hematopoietic stem cell function in vivo is uniquely high in the CXB-12 
      mouse strain.
PG  - 4124-31
AB  - Bone marrow cells (BMCs) from CXB-12/HiaJ (CXB-12) mice had 14 times the total 
      long-term repopulating ability found in the best of 11 other CXB recombinant 
      inbred (RI) lines. BMCs from each RI line donor were mixed with genetically 
      marked standard competitor BMCs from the BALB/cByxC57BL/6 F1 (CByB6F1) hybrid, 
      the mice used to produce the RI lines, and the mixtures repopulated lethally 
      irradiated CByB6F1 recipients. Percentages of donor-type erythrocytes and 
      lymphocytes measured the actual long-term repopulating functions of the donor RI 
      lines relative to the standard competitor. CXB-12 BMCs repopulated better after 3 
      or 6 months than after 1 month, suggesting that the most primitive precursors 
      were involved. Compared to CByB6F1 standard competitor cells, CXB-12 cells 
      repopulated 3 to 12 times as well, with their advantage increasing when higher 
      doses of cells were transplanted, probably because of hybrid resistance of the 
      recipient against low doses. This was far better than expected, because F1 cells 
      normally function 2 to 3 times as well as cells from an inbred strain. In 
      competitive dilution, the advantage resulted from 2 factors: more precursor cells 
      and more function per precursor. In the model that best fit the data, CXB-12 
      donors had 2.4 times the concentration of hematopoietic stem cells (HSCs) as the 
      CByB6F1 standard, and each HSC repopulated 1.4 times as well. CXB-12 mice did not 
      have elevated erythrocyte and lymphocyte numbers in blood and marrow and did not 
      have unusually elevated concentrations of colony-forming unit spleen, cobblestone 
      colonies, and long-term colony-initiating cells in marrow.
FAU - Chen, J
AU  - Chen J
AD  - The Jackson Laboratory, Bar Harbor, ME 04609, USA.
FAU - Astle, C M
AU  - Astle CM
FAU - Muller-Sieburg, C E
AU  - Muller-Sieburg CE
FAU - Harrison, D E
AU  - Harrison DE
LA  - eng
GR  - DK-48015/DK/NIDDK NIH HHS/United States
GR  - HL-58705/HL/NHLBI NIH HHS/United States
GR  - HL-58820/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Blood Cell Count
MH  - Bone Marrow Transplantation
MH  - Cell Count
MH  - Cell Division
MH  - Cells, Cultured
MH  - Cellular Senescence
MH  - Colony-Forming Units Assay
MH  - Crosses, Genetic
MH  - Graft Survival
MH  - *Hematopoiesis/genetics
MH  - Hematopoietic Stem Cells/*physiology
MH  - Hybridization, Genetic
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains/genetics/*physiology
MH  - Radiation Chimera
EDAT- 2000/12/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/09 11:00
PHST- 2000/12/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/09 11:00 [entrez]
AID - S0006-4971(20)48164-9 [pii]
PST - ppublish
SO  - Blood. 2000 Dec 15;96(13):4124-31.