PMID- 11110711
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 13
DP  - 2000 Dec 15
TI  - Similar pattern of thymic-dependent T-cell reconstitution in infants with severe 
      combined immunodeficiency after human leukocyte antigen (HLA)-identical and 
      HLA-nonidentical stem cell transplantation.
PG  - 4344-9
AB  - Donor T cells after stem cell transplantation reconstitute by 2 different 
      pathways: by expansion from grafted, mature T cells and by intrathymic maturation 
      from progenitor cells. This study characterized thymic-dependent reconstitution 
      of CD4(+) T cells following different transplant modalities in patients with 
      severe combined immunodeficiency (SCID). Three groups of patients were studied: 
      one group after transplantation from human leukocyte antigen (HLA)-identical 
      siblings with unmanipulated grafts without conditioning, a second group after 
      transplantation from HLA-nonidentical parents with T-cell-depleted grafts without 
      preconditioning, and a third group with prior conditioning. Reconstitution of the 
      T-cell compartment was monitored by determining the expression of CD45 isoforms 
      by developing CD4(+) cells in the peripheral blood and in discriminating expanded 
      (CD45RO(+)) and newly generated (CD45RA(+)) T cells. Concomitantly, changes in 
      the size of the thymus were evaluated sequentially by ultrasonography. 
      Reconstitution of CD4(+)CD45RA(+) cells was delayed in all patients for several 
      months, including patients after HLA-identical transplantation, and was always 
      paralleled by normalization of the size of the thymus. No engraftment of donor 
      progenitor cells was observed, as studied in one patient transplanted without 
      conditioning. CD4(+)CD45RO(+) cells were detected early after transplantation 
      only in patients given unmanipulated grafts. The study showed that 
      thymic-dependent T-cell maturation in these patients with SCID runs an autonomous 
      course, independent of graft manipulation, of major HLA disparities, and of 
      whether conditioning is used or not. In addition, thymic maturation may not 
      require engraftment of donor-derived CD34(+) cells in the marrow. (Blood. 
      2000;96:4344-4349)
FAU - Muller, S M
AU  - Muller SM
AD  - Department of Pediatrics, University Hospital of Ulm, Ulm, Germany.
FAU - Kohn, T
AU  - Kohn T
FAU - Schulz, A S
AU  - Schulz AS
FAU - Debatin, K M
AU  - Debatin KM
FAU - Friedrich, W
AU  - Friedrich W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (HLA Antigens)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/cytology
MH  - Child
MH  - Child, Preschool
MH  - Chimera
MH  - Female
MH  - Graft Survival
MH  - Granulocyte Colony-Stimulating Factor/pharmacology
MH  - HLA Antigens/*genetics
MH  - *Hematopoiesis
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility
MH  - Humans
MH  - Infant
MH  - Leukocyte Common Antigens/analysis
MH  - Male
MH  - Severe Combined Immunodeficiency/*therapy
MH  - T-Lymphocyte Subsets/*cytology
MH  - Thymus Gland/*cytology/diagnostic imaging
MH  - Time Factors
MH  - Transplantation Conditioning
MH  - Ultrasonography
EDAT- 2000/12/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/09 11:00
PHST- 2000/12/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/09 11:00 [entrez]
AID - S0006-4971(20)48192-3 [pii]
PST - ppublish
SO  - Blood. 2000 Dec 15;96(13):4344-9.