PMID- 11112142
OWN - NLM
STAT- MEDLINE
DCOM- 20010126
LR  - 20061115
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 162
IP  - 6
DP  - 2000 Dec
TI  - Eosinophilic granulocytes and interleukin-6 level in bronchoalveolar lavage fluid 
      are associated with the development of obliterative bronchiolitis after lung 
      transplantation.
PG  - 2221-5
AB  - In a prospective cohort study, we assessed whether changes in total cell counts 
      and differentiation and interleukin-6 (IL-6), IL-8, and monocyte chemoattractant 
      protein-1 (MCP-1) concentrations in bronchoalveolar lavage fluid (BALF) are 
      associated with a higher risk to develop obliterative bronchiolitis (OB). We 
      investigated 60 lung transplant patients (follow-up of 2 to 8 yr) with either 
      histologic evidence of OB within 1 yr after lung transplantation (n = 19) or no 
      pathology, good outcome (GO) for at least 24 mo and well-preserved lung function, 
      i.e., FEV > or = 80% of baseline (n = 41). Median time between lung 
      transplantation and the first BAL was 42 d for the GO group and 41 d for the OB 
      group (p > 0.05). In the bronchial fraction, median total cell counts (0.06 x 
      10(3)/ml versus 0.04 x 10(3)/ml), lymphocyte (9 x 10(3)/ml versus 2 x 10(3)/ml), 
      and eosinophilic granulocyte counts (1 x 10(3)/ml versus 0) were significantly 
      higher in the OB group than in the GO group (p < 0.05). In the alveolar fraction, 
      this was the case for the median value of neutrophilic granulocyte counts (19 x 
      10(3)/ml versus 4 x 10(3)/ml), respectively. Median values of IL-6 and IL-8 
      concentrations in both bronchial (IL-6: 23 versus 6 pg/ml, IL-8: 744 versus 102 
      pg/ml) and alveolar fractions (IL-6: 13 versus 3 pg/ml, IL-8: 110 versus 30 
      pg/ml) of the BALF were significantly higher in the OB group than in the GO 
      group. By means of logistic regression, we showed that higher total cell, 
      neutrophilic granulocyte, and lymphocyte counts, the presence of eosinophilic 
      granulocytes, and higher concentrations of IL-6 and IL-8 were significantly 
      associated with an increased risk to develop OB. We conclude that monitoring cell 
      counts, neutrophilic and eosinophilic granulocytes, IL-6, and IL-8 in BALF within 
      2 mo after lung transplantation in addition to the transbronchial lung biopsy 
      (TBB) pathology will contribute to a better identification and management of the 
      group of patients at risk for developing OB within a year.
FAU - Scholma, J
AU  - Scholma J
AD  - Department of Pulmonology, University Hospital Groningen, University of 
      Groningen, The Netherlands. j.scholma@int.azg.nl
FAU - Slebos, D J
AU  - Slebos DJ
FAU - Boezen, H M
AU  - Boezen HM
FAU - van den Berg, J W
AU  - van den Berg JW
FAU - van der Bij, W
AU  - van der Bij W
FAU - de Boer, W J
AU  - de Boer WJ
FAU - Koeter, G H
AU  - Koeter GH
FAU - Timens, W
AU  - Timens W
FAU - Kauffman, H F
AU  - Kauffman HF
FAU - Postma, D S
AU  - Postma DS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Adult
MH  - Bronchiolitis Obliterans/etiology/metabolism/*pathology
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Chemokine CCL2/analysis
MH  - Chi-Square Distribution
MH  - Cohort Studies
MH  - Eosinophils/*pathology
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Interleukin-6/*analysis
MH  - Interleukin-8/analysis
MH  - Leukocyte Count/statistics & numerical data
MH  - Logistic Models
MH  - Lung Transplantation/*adverse effects/pathology/physiology
MH  - Male
MH  - Postoperative Complications/etiology/metabolism/*pathology
MH  - Prospective Studies
MH  - Statistics, Nonparametric
MH  - Time Factors
EDAT- 2000/12/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/09 11:00
PHST- 2000/12/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/09 11:00 [entrez]
AID - 10.1164/ajrccm.162.6.9911104 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2000 Dec;162(6):2221-5. doi: 
      10.1164/ajrccm.162.6.9911104.