PMID- 11112152
OWN - NLM
STAT- MEDLINE
DCOM- 20010126
LR  - 20171116
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 162
IP  - 6
DP  - 2000 Dec
TI  - Halothane reduces the early lipopolysaccharide-induced lung inflammation in 
      mechanically ventilated rats.
PG  - 2278-86
AB  - Several studies suggest that anesthetics modulate the immune response. The aim of 
      this study was to investigate the effect of halothane and thiopental on the lung 
      inflammatory response. Rats submitted or not to intratracheal (IT) instillation 
      of lipopolysaccharides (LPS) were anesthetized with either halothane (0. 5, 1, or 
      1.5%) or thiopental (60 mg. kg(-1)) and mechanically ventilated for 4 h. Control 
      rats were treated or not by LPS without anesthesia. Lung inflammation was 
      assessed by total and differential cell counts in bronchoalveolar lavage fluids 
      (BALF) and by cytokine measurements (tumor necrosis factor-alpha [TNF-alpha], 
      interleukin-6 [IL-6], macrophage inflammatory protein-2 [MIP-2], and monocyte 
      chemoattractant protein-1 [MCP-1]) in BALF and lung homogenates. In the absence 
      of LPS treatment, neither halothane nor thiopental modified the moderate 
      inflammatory response induced by tracheotomy or mechanical ventilation. Cell 
      recruitment and cytokine concentrations were increased in all groups receiving IT 
      LPS. However, in halothane-anesthetized rats (halothane > or = 1%), but not in 
      thiopental-anesthetized rats, the LPS-induced lung inflammation was altered in a 
      dose-dependent manner. Indeed, when using 1% halothane, polymorphonuclear 
      leukocyte (PMN) recruitment was decreased by 55% (p < 0.001) and TNF-alpha, IL-6, 
      and MIP-2 concentrations in BALF and lung homogenates were decreased by more than 
      60% (p < 0.001) whereas total protein and MCP-1 concentrations remained 
      unchanged. The decrease of MIP-2 (observed at the protein and messenger RNA 
      [mRNA] level) was strongly correlated to the decrease of PMN recruitment (r = 
      0.73, p < 0.05). This halothane-reduced lung inflammatory response was transient 
      and was reversed 20 h after the end of the anesthesia. Our study shows that 
      halothane > or = 1%, delivered during 4 h by mechanical ventilation, but not 
      mechanical ventilation per se, alters the early LPS-induced lung inflammation in 
      the rat, suggesting a specific effect of halothane on this response.
FAU - Giraud, O
AU  - Giraud O
AD  - Unite INSERM 408, Faculte de Medecine Xavier Bichat, Paris, France.
FAU - Seince, P F
AU  - Seince PF
FAU - Rolland, C
AU  - Rolland C
FAU - Lecon-Malas, V
AU  - Lecon-Malas V
FAU - Desmonts, J M
AU  - Desmonts JM
FAU - Aubier, M
AU  - Aubier M
FAU - Dehoux, M
AU  - Dehoux M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Anesthetics, Intravenous)
RN  - 0 (Lipopolysaccharides)
RN  - JI8Z5M7NA3 (Thiopental)
RN  - UQT9G45D1P (Halothane)
SB  - IM
MH  - Anesthetics, Inhalation/administration & dosage/*pharmacology
MH  - Anesthetics, Intravenous/administration & dosage/pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Escherichia coli
MH  - Halothane/administration & dosage/*pharmacology
MH  - Lipopolysaccharides
MH  - Lung/chemistry/drug effects/pathology
MH  - Male
MH  - Molecular Sequence Data
MH  - Pneumonia/chemically induced/*metabolism/pathology
MH  - Polymerase Chain Reaction/methods/statistics & numerical data
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Respiration, Artificial
MH  - Specific Pathogen-Free Organisms
MH  - Thiopental/administration & dosage/pharmacology
MH  - Time Factors
EDAT- 2000/12/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/09 11:00
PHST- 2000/12/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/09 11:00 [entrez]
AID - 10.1164/ajrccm.162.6.9807113 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2000 Dec;162(6):2278-86. doi: 
      10.1164/ajrccm.162.6.9807113.