PMID- 11112425
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 279
IP  - 1
DP  - 2000 Dec 9
TI  - Regulation of HGF/SF gene expression in MRC-5 cells by N-acetylcysteine.
PG  - 108-15
AB  - The effect of N-acetylcysteine (NAC) on levels of hepatocyte growth 
      factor/scatter factor (HGF/SF) gene transcripts was investigated in the human 
      lung embryonic fibroblast cell line, MRC-5. NAC increased expression of HGF/SF 
      mRNA, in a dose- and time-dependent fashion, by a mechanism independent of 
      glutathione synthesis but sensitive to oxidant stress induced by H(2)O(2). Using 
      actinomycin D to block RNA synthesis, it was observed that NAC had no effect on 
      the stability of the HGF/SF mRNA transcripts. NAC increased HGF/SF promoter 
      activity in cells transiently transfected with chloramphenicol acetyltransferase 
      (CAT) reporter genes driven by HGF/SF gene 5'-flanking sequences. Primer 
      extension analysis demonstrated that NAC enhanced the expression of HGF/SF mRNA 
      transcribed from the main transcription initiation site. Although the 5' flanking 
      region of the HGF/SF gene contains a sequence at -1019 to -1011 with homology to 
      the NF-kappaB response element, electrophoretic mobility shift assay demonstrated 
      that this site did not bind nuclear factors in MRC-5 cells in the presence or 
      absence of NAC. In contrast to the effect on HGF/SF mRNA, NAC did not increase 
      HGF/SF protein production by MRC-5 cells.
CI  - Copyright 2000 Academic Press.
FAU - Harrison, P M
AU  - Harrison PM
AD  - Institute of Liver Studies, GKT School of Medicine, King's Denmark Hill Campus, 
      Bessemer Road, London, SE5 9PJ, United Kingdom. phillip.harrison@kcl.ac.uk
FAU - Farzaneh, F
AU  - Farzaneh F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - DNA Primers
MH  - Electrophoresis/methods
MH  - Gene Expression Regulation/*drug effects
MH  - Hepatocyte Growth Factor/*genetics
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
EDAT- 2000/12/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/09 11:00
PHST- 2000/12/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/09 11:00 [entrez]
AID - S0006-291X(00)93904-4 [pii]
AID - 10.1006/bbrc.2000.3904 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Dec 9;279(1):108-15. doi: 
      10.1006/bbrc.2000.3904.