PMID- 11112501
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 278
IP  - 1
DP  - 2000 Dec 5
TI  - Co-replication of a reovirus and a polydnavirus in the ichneumonid parasitoid 
      Hyposoter exiguae.
PG  - 266-75
AB  - A recently established colony of the ichneumonid parasitoid, Hyposoter exiguae, 
      was found to carry both a reovirus (HeRV) and a polydnavirus (HePDV). 
      Morphogenesis of these viruses was observed in all cells comprising the ovarian 
      calyx epithelium, apparently without detrimental effect to the parasitoid. While 
      polydnavirus replication in H. exiguae was restricted to the calyx region, HeRV 
      was detected in ovarioles, oviducts, midguts, malpighian tubules, and accessory 
      glands associated with the male reproductive system. In addition, HeRV was able 
      to infect the fat body of parasitized host larvae and to establish a persistent 
      infection in vitro. Electron microscopy revealed that both viruses were released 
      into the calyx fluid compartment exclusively by budding, a phenomenon rarely 
      observed among the Reoviridae; HeRV envelopes thus obtained, however, appeared to 
      be subsequently shed within the oviducts. HeRV particles were concentrated to 
      near homogeneity by differential centrifugation; mature virions consisted of 
      seven to eight structural polypeptides and 10 dsRNA genome segments. Prominent 
      spikes were observed at the vertices of icosahedral core particles. Most, but not 
      all, individuals comprising the H. exiguae colony appeared to be infected with 
      HeRV, suggesting a commensal relationship between wasp and virus; however, while 
      this association is of obvious benefit to the virus, it seems unlikely that any 
      advantage accrues to the parasitoid which carries it.
CI  - Copyright 2000 Academic Press.
FAU - Stoltz, D
AU  - Stoltz D
AD  - Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova 
      Scotia, B3H 4H7, Canada. dstoltz@is.dal.ca
FAU - Makkay, A
AU  - Makkay A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Microscopy, Electron
MH  - Ovary/virology
MH  - *Polydnaviridae
MH  - RNA, Double-Stranded/analysis
MH  - Reoviridae/genetics/*isolation & purification/ultrastructure
MH  - Viral Proteins/analysis
MH  - Wasps/*virology
EDAT- 2000/12/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/09 11:00
PHST- 2000/12/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/09 11:00 [entrez]
AID - S0042-6822(00)90652-4 [pii]
AID - 10.1006/viro.2000.0652 [doi]
PST - ppublish
SO  - Virology. 2000 Dec 5;278(1):266-75. doi: 10.1006/viro.2000.0652.