PMID- 11113702
OWN - NLM
STAT- MEDLINE
DCOM- 20010126
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 472
IP  - 1-2
DP  - 2000 Dec 20
TI  - The genotoxicity of 3-nitrobenzanthrone and the nitropyrene lactones in human 
      lymphoblasts.
PG  - 93-103
AB  - Polycyclic aromatic hydrocarbons (PAH) and nitrated polycyclic aromatic compounds 
      (nitro-PAC) have been found to be mutagenic in bacterial and human cells as well 
      as carcinogenic in rodents. In this investigation, the genotoxic effects of 
      3-nitrobenzanthrone (3NB) and a mixture of nitropyrene lactones (NPLs) were 
      determined using forward mutation assays performed in two human B-lymphoblastoid 
      cell lines, MCL-5 and h1A1v2, which are responsive to the nitro-PAC class of 
      compounds. Mutagenicity of the compounds was determined at the heterozygous tk 
      locus and the hemizygous hprt locus, thus, identifying both large-scale loss of 
      heterozygosity (LOH) events as well as intragenic mutagenic events. Genotoxicity 
      was also determined using the CREST modified micronucleus assay, which detects 
      chromosomal loss and breakage events. Results indicate 3NB is an effective human 
      cell mutagen, significantly inducing mutations at the tk and hprt loci in both 
      cell lines, and inducing micronuclei in the h1A1v2 cell line. The NPL isomers are 
      also mutagenic, inducing mutations at the two loci as well as micronuclei in both 
      cell lines. Because of their mutagenic potencies and their presence in ambient 
      air, further assessments should be made of human exposures to these nitro-PAC and 
      the potential health risks involved.
FAU - Phousongphouang, P T
AU  - Phousongphouang PT
AD  - Environmental Toxicology Graduate Program, Air Pollution Research Center, 
      University of California, Riverside, CA 92521, USA.
FAU - Grosovsky, A J
AU  - Grosovsky AJ
FAU - Eastmond, D A
AU  - Eastmond DA
FAU - Covarrubias, M
AU  - Covarrubias M
FAU - Arey, J
AU  - Arey J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Benz(a)Anthracenes)
RN  - 0 (Lactones)
RN  - 0 (Mutagens)
RN  - 0 (Pyrenes)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
RN  - EC 2.7.1.21 (Thymidine Kinase)
RN  - O4OJW7BC7W (3-nitrobenzanthrone)
RN  - Q41OR9510P (Melphalan)
SB  - IM
MH  - B-Lymphocytes/cytology/*drug effects
MH  - Benz(a)Anthracenes/*toxicity
MH  - Benzo(a)pyrene/toxicity
MH  - Cell Line
MH  - Humans
MH  - Hypoxanthine Phosphoribosyltransferase/genetics
MH  - Lactones/*toxicity
MH  - *Loss of Heterozygosity
MH  - Male
MH  - Melphalan/toxicity
MH  - Mutagenicity Tests
MH  - Mutagens/*toxicity
MH  - Pyrenes/*toxicity
MH  - Thymidine Kinase/genetics
EDAT- 2000/12/13 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/13 11:00
PHST- 2000/12/13 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/13 11:00 [entrez]
AID - S1383571800001352 [pii]
AID - 10.1016/s1383-5718(00)00135-2 [doi]
PST - ppublish
SO  - Mutat Res. 2000 Dec 20;472(1-2):93-103. doi: 10.1016/s1383-5718(00)00135-2.