PMID- 11114257
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20071114
IS  - 0969-9961 (Print)
IS  - 0969-9961 (Linking)
VI  - 7
IP  - 6 Pt B
DP  - 2000 Dec
TI  - Brain-derived neurotrophic factor in astrocytes, oligodendrocytes, and 
      microglia/macrophages after spinal cord injury.
PG  - 574-85
AB  - Recent studies suggest that the injured adult spinal cord responds to 
      brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) with enhanced 
      neuron survival and axon regeneration. Potential neurotrophin sources and 
      cellular localization in spinal cord are largely undefined. We examined glial 
      BDNF localization in normal cord and its temporospatial distribution after injury 
      in vivo. We used dual immunolabeling for BDNF and glial fibrillary acidic protein 
      (GFAP) in astrocytes, adenomatous polyposis coli tumor suppressor protein (APC) 
      for oligodendrocytes or type III CDH receptor (OX42) for microglia/macrophages. 
      In normal cord, small subsets of astrocytes and microglia/macrophages and most 
      oligodendrocytes exhibited BDNF-immunoreactivity. Following injury, the number of 
      BDNF-immunopositive astrocytes and microglia/macrophages increased dramatically 
      at the injury site over time. Most oligodendrocytes contained BDNF 1 day and 1 
      week following injury, but APC-positive cells were largely absent at the injury 
      site 6 weeks postinjury. Glial BDNF-immunolabeling was also examined 10 and 20 mm 
      from the wound. Ten millimeters from the lesion, astrocyte and 
      microglia/macrophage BDNF-immunolabeling resembled that at the injury at all 
      times examined. Twenty millimeters from injury, BDNF localization in all three 
      glial subtypes resembled controls, regardless of time postlesion. Our findings 
      suggest that in normal adult cord, astrocytes, oligodendrocytes, and 
      microglia/macrophages play roles in local trophin availability and in 
      trophin-mediated injury and healing responses directly within and surrounding the 
      wound site.
CI  - Copyright 2000 Academic Press.
FAU - Dougherty, K D
AU  - Dougherty KD
AD  - Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical 
      School, Piscataway, New Jersey 08854, USA.
FAU - Dreyfus, C F
AU  - Dreyfus CF
FAU - Black, I B
AU  - Black IB
LA  - eng
GR  - HD23315/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Adenomatous Polyposis Coli Protein)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Adenomatous Polyposis Coli Protein
MH  - Animals
MH  - Antibody Specificity
MH  - Astrocytes/cytology/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Count
MH  - Cytoskeletal Proteins/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - Macrophages/cytology/metabolism
MH  - Microglia/cytology/*metabolism
MH  - Oligodendroglia/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/cytology/metabolism/pathology
MH  - Spinal Cord Injuries/*metabolism/pathology
MH  - Time Factors
EDAT- 2000/12/15 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/15 11:00
PHST- 2000/12/15 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/15 11:00 [entrez]
AID - S0969-9961(00)90318-8 [pii]
AID - 10.1006/nbdi.2000.0318 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2000 Dec;7(6 Pt B):574-85. doi: 10.1006/nbdi.2000.0318.