PMID- 11114283
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20190503
IS  - 0003-4967 (Print)
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 60
IP  - 1
DP  - 2001 Jan
TI  - Decline after immobilisation and recovery after remobilisation of synovial fluid 
      IL1, TIMP, and chondroitin sulphate levels in young beagle dogs.
PG  - 55-60
AB  - OBJECTIVE: To monitor the concentration of markers of cartilage and synovium 
      metabolism in the knee (stifle) joint synovial fluid of young beagles subjected 
      to immobilisation and subsequent remobilisation. METHODS: The right hind limb of 
      17 dogs was immobilised in flexion for 11 weeks. Simultaneously, the 
      contralateral left knee was exposed to increased weight bearing. The 
      remobilisation period lasted 50 weeks. Litter mates served as controls. The 
      concentration in joint lavage fluid of interleukin 1alpha (IL1alpha) was measured 
      by immunoassay, the activity of phospholipase A(2) (PLA(2)) was determined by an 
      extraction method, chondroitin sulphate (CS) concentration by precipitation with 
      Alcian blue, hyaluronan (HA) by an ELISA-like assay using biotinylated HA-binding 
      complexes, matrix metalloproteinase 3 (MMP-3), and tissue inhibitor of 
      metalloproteinases 1 (TIMP-1) by sandwich ELISA, and synovitis was scored by 
      light microscopy. RESULTS: Synovitis or effusion was absent in all experimental 
      and control groups. Immobilisation decreased the joint lavage fluid levels of 
      IL1alpha (p<0.05), TIMP (p< 0.05), and the concentration of CS down to 38% 
      (p<0.05) in comparison with untreated litter mates with normal weight bearing. 
      Immobilisation did not affect the activity of PLA(2), or the concentration of 
      MMP-3 or HA in synovial fluid. Joint remobilisation restored the decreased 
      concentrations of markers to control levels. Increased weight bearing did not 
      change the concentrations of markers in comparison with the control joints with 
      normal weight bearing. CONCLUSIONS: 11 weeks' joint immobilisation decreased the 
      concentration of markers of cartilage and synovium metabolism in the synovial 
      fluid, and remobilisation restored the concentrations to control levels. The 
      changes in joint metabolism induced by immobilisation, as reflected by the 
      markers, are thus different from those found in osteoarthritis, where increased 
      levels of these markers are associated with enhanced degradation and synthesis. 
      These findings suggest that the change induced in joint metabolism by 
      immobilisation is reversible in its early stages.
FAU - Haapala, J
AU  - Haapala J
AD  - Department of Surgery, Kuopio University Hospital, Finland. Jussi.Haapala@phks.fi
FAU - Arokoski, J P
AU  - Arokoski JP
FAU - Ronkko, S
AU  - Ronkko S
FAU - Agren, U
AU  - Agren U
FAU - Kosma, V M
AU  - Kosma VM
FAU - Lohmander, L S
AU  - Lohmander LS
FAU - Tammi, M
AU  - Tammi M
FAU - Helminen, H J
AU  - Helminen HJ
FAU - Kiviranta, I
AU  - Kiviranta I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Biomarkers)
RN  - 0 (Interleukin-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 9007-28-7 (Chondroitin Sulfates)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Cartilage, Articular/*metabolism
MH  - Chondroitin Sulfates/metabolism
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Immobilization/*physiology
MH  - Interleukin-1/metabolism
MH  - Movement/physiology
MH  - Synovial Fluid/*metabolism
MH  - Synovitis/etiology
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Weight-Bearing/physiology
PMC - PMC1753360
EDAT- 2000/12/15 11:00
MHDA- 2001/03/03 10:01
PMCR- 2004/01/01
CRDT- 2000/12/15 11:00
PHST- 2000/12/15 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/15 11:00 [entrez]
PHST- 2004/01/01 00:00 [pmc-release]
AID - 10.1136/ard.60.1.55 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2001 Jan;60(1):55-60. doi: 10.1136/ard.60.1.55.