PMID- 11114860
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20191104
IS  - 1340-6868 (Print)
IS  - 1340-6868 (Linking)
VI  - 7
IP  - 4
DP  - 2000
TI  - Diagnosis of ductal carcinoma in situ (DCIS) and intraductal papilloma using 
      fluorescence in situ hybridization (FISH) analysis.
PG  - 332-6
AB  - BACKGROUND: It is often difficult to pre-operatively diagnose ductal carcinoma in 
      situ (DCIS)or intraductal papilloma (IDP). Current reports show that breast 
      cancer frequently has numerical aberrations of chromosomes 1, 11 and 17. We 
      investigated whether fluorescence in situ hybridization (FISH) analysis using 
      three centromere-specific probes for chromosomes 1, 11 and 17 was feasible for 
      diagnosing intraductal breast lesions. METHODS: Fine-needle aspiration specimens 
      from 102 breast lesions including DCIS (10), invasive ductal carcinoma (IDC) 
      (78), IDP (7), fibroadenoma (6) and mastopathy (1) were examined for numerical 
      aberrations on chromosomes 1, 11, 17 using FISH. If over 15% of all cells showed 
      one signal, the sample was judged monosomic. If over 20% of cells showed three or 
      more signals, it was considered polysomic. If the specimen had an aberration of 
      at least one chromosome, it was judged positive. RESULTS: Nine of 10 DCISs showed 
      numerical aberrations of at least one chromosome whereas 65 of 78 IDCs and 2 of 
      14 benign lesions (containing 7 IDPs of which one case was positive) showed 
      numerical aberrations on these chromosomes. The proportion of positive results 
      was highest with DCIS. Moreover 6 out of 7 DCISs showed an aberration of all 
      three chromosomes simultaneously and one case showed an aberration of two 
      chromosomes. All aberrations in case of DCIS were polysomic while two benign 
      lesions and 15 IDCs showed a monosomic pattern. CONCLUSION: FISH may enable more 
      accurate diagnosis of intraductal breast lesions.
FAU - Komoike, Y
AU  - Komoike Y
AD  - Department of Surgery, Osaka Medical Center for Cancer and Cardiovascular 
      Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
FAU - Motomura, K
AU  - Motomura K
FAU - Inaji, H
AU  - Inaji H
FAU - Koyama, H
AU  - Koyama H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Breast Cancer
JT  - Breast cancer (Tokyo, Japan)
JID - 100888201
SB  - IM
MH  - Breast Neoplasms/*diagnosis/genetics/pathology
MH  - Carcinoma in Situ/*diagnosis/genetics/pathology
MH  - Carcinoma, Ductal, Breast/*diagnosis/genetics/pathology
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Monosomy
MH  - Papilloma/*diagnosis/genetics/pathology
EDAT- 2000/12/15 11:00
MHDA- 2001/05/18 10:01
CRDT- 2000/12/15 11:00
PHST- 2000/12/15 11:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2000/12/15 11:00 [entrez]
AID - 10.1007/BF02966400 [doi]
PST - ppublish
SO  - Breast Cancer. 2000;7(4):332-6. doi: 10.1007/BF02966400.