PMID- 11119150
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20190915
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 89
IP  - 5
DP  - 2000 Nov
TI  - Microbial succession during a composting process as evaluated by denaturing 
      gradient gel electrophoresis analysis.
PG  - 768-77
AB  - Microbial succession during a laboratory-scale composting process of garbage was 
      analysed by denaturing gradient gel electrophoresis (DGGE) combined with 
      measurement of physicochemical parameters such as temperature, pH, organic acids, 
      total dissolved organic carbon and water-soluble humic substance. From the 
      temperature changes, a rapid increase from 25 to 58 degrees C and then a gradual 
      decrease, four phases were recognized in the process as follows; mesophilic (S), 
      thermophilic (T), cooling (C) and maturing (M). The polymerase chain 
      reaction-amplified 16S rDNA fragments with universal (907R) and eubacterial (341F 
      with GC clamp) primers were subjected to DGGE analysis. Consequently, the DGGE 
      band pattern changed during the composting process. The direct sequences from 
      DGGE bands were related to those of known genera in the DNA database. The 
      microbial succession determined by DGGE was summarized as follows: in the S phase 
      some fermenting bacteria, such as lactobacillus, were present with the existing 
      organic acids; in the T phase thermophilic bacillus appeared and, after the C 
      phase, bacterial populations were more complex than in previous phases and the 
      phylogenetic positions of those populations were relatively distant from strains 
      so far in the DNA database. Thus, the DGGE method is useful to reveal microbial 
      succession during a composting process.
FAU - Ishii, K
AU  - Ishii K
AD  - Department of Biological Science, Graduate School of Science, Tokyo Metropolitan 
      University, Hachiohji, Japan.
FAU - Fukui, M
AU  - Fukui M
FAU - Takii, S
AU  - Takii S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*genetics/growth & development
MH  - DNA, Bacterial/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/analysis
MH  - *Refuse Disposal
MH  - Temperature
EDAT- 2000/12/19 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/19 11:00
PHST- 2000/12/19 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/19 11:00 [entrez]
AID - jam1177 [pii]
AID - 10.1046/j.1365-2672.2000.01177.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2000 Nov;89(5):768-77. doi: 10.1046/j.1365-2672.2000.01177.x.