PMID- 11119603
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20181130
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 75
IP  - 1
DP  - 2001 Jan
TI  - Interferon regulatory factor 7 mediates activation of Tap-2 by Epstein-Barr virus 
      latent membrane protein 1.
PG  - 341-50
AB  - Transporter associated with antigen processing 2 (Tap-2) is responsible for 
      ATP-dependent transport of peptides from the cytosol to the endoplasmic 
      reticulum, where peptides bind to newly synthesized human leukocyte antigen (HLA) 
      class I molecules, which are essential for cellular immune responses. 
      Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1) has been shown to 
      induce the expression of Tap-2. In this study, the induction of endogenous Tap-2 
      by LMP-1 is shown to be associated with and requires the expression of interferon 
      regulatory factor 7 (IRF-7). In DG75 Burkitt's lymphoma (BL) cells, in which 
      LMP-1 induces the expression of IRF-7, LMP-1 induced endogenous Tap-2, and 
      ectopic expression of IRF-7 could enhance the induction. In Akata BL cells, in 
      which LMP-1 could not induce IRF-7, LMP-1 could not induce Tap-2. Addition of 
      IRF-7, which complements the defect in Akata cells, could stimulate the 
      expression of Tap-2. Furthermore, LMP-1 and IRF-7A but not other IRF-7 splicing 
      variants could activate endogenous Tap-2. A Tap-2 promoter reporter construct 
      could be activated by the overexpression of IRF-7A. The activation could be 
      specifically enhanced by LMP-1 and was dependent on an intact 
      interferon-stimulated response element (ISRE) present in the Tap-2 promoter. 
      Also, IRF-7 can bind to the Tap-2 promoter under physiological conditions in 
      vivo, as shown by formaldehyde cross-linking, as well as to the Tap-2 ISRE in 
      vitro, as shown by gel mobility shift assays. Furthermore, LMP-1 facilitates the 
      phosphorylation and nuclear translocation of IRF-7. These data point to the role 
      of IRF-7 as a secondary mediator of LMP-1-activated signal transduction for Tap-2 
      as follows: LMP-1 stimulates the expression of IRF-7 and facilitates its 
      phosphorylation and nuclear translocation, and then the activated IRF-7 mediates 
      the activation of the cellular Tap-2 gene. The induction of Tap-2 by IRF-7 and 
      LMP-1 may have an important implication for the immune response to EBV and its 
      persistence in vivo.
FAU - Zhang, L
AU  - Zhang L
AD  - Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel 
      Hill, North Carolina 27599-7265, USA. luzhang@med.unc.edu
FAU - Pagano, J S
AU  - Pagano JS
LA  - eng
GR  - P01 CA019014/CA/NCI NIH HHS/United States
GR  - AI 42372-01/AI/NIAID NIH HHS/United States
GR  - CA 19014/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 3)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (IRF7 protein, human)
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (PDLIM7 protein, human)
RN  - 145892-13-3 (TAP2 protein, human)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 3
MH  - ATP-Binding Cassette Transporters/*genetics
MH  - Adaptor Proteins, Signal Transducing
MH  - Carrier Proteins/analysis/*physiology
MH  - Cell Nucleus/metabolism
MH  - Cytoskeletal Proteins
MH  - DNA-Binding Proteins/analysis/*physiology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interferon Regulatory Factor-7
MH  - Intracellular Signaling Peptides and Proteins
MH  - LIM Domain Proteins
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
PMC - PMC113927
EDAT- 2000/12/19 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/01/01
CRDT- 2000/12/19 11:00
PHST- 2000/12/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/19 11:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 1052 [pii]
AID - 10.1128/JVI.75.1.341-350.2001 [doi]
PST - ppublish
SO  - J Virol. 2001 Jan;75(1):341-50. doi: 10.1128/JVI.75.1.341-350.2001.