PMID- 11121898
OWN - NLM
STAT- MEDLINE
DCOM- 20030714
LR  - 20191104
IS  - 0378-4320 (Print)
IS  - 0378-4320 (Linking)
VI  - 64
IP  - 3-4
DP  - 2000 Dec 29
TI  - Kinase pathways in dominant and subordinate ovarian follicles during the first 
      wave of follicular development in sheep.
PG  - 221-31
AB  - The mechanism by which one or more dominant ovarian follicles continue 
      development while other subordinate follicles regress is not known. The mitogen 
      activated protein kinases (MAPKs) are a group of kinases that are activated by 
      hormonal factors and form a cascade of processes that regulate cell growth, 
      division and differentiation. The aim of the present experiment was to 
      characterise the presence of the MAPKs, Erk 1/Erk 2 and Akt in healthy dominant 
      follicles and regressing subordinate follicles. Following in vivo monitoring of 
      ovarian follicle development, three ewes were ovariectomised and the follicular 
      fluid and follicle wall (theca and granulosa cells) saved from the dominant and 
      largest subordinate follicle. The dissected diameter and follicular fluid 
      oestradiol concentration of the dominant follicle was larger (P<0.01) than the 
      largest subordinate follicle (6.5+/-0.0mm and 41.3+/-4.9ng/ml versus 4.7+/-0.3mm 
      and 0.6+/-0.4ng/ml). Western blot analyses showed that there was more Akt 
      (202.7+/-6.4 versus 59.6+/-32.7 units; P<0.05) and Erk 1/Erk 2 (104.5+/-10.6 
      versus 0.3+/-0.2 units; P<0.01) present in follicle wall samples from the 
      dominant compared to the largest subordinate follicles. Phosphorylated forms of 
      Akt and Erk 1/Erk 2 were detected in samples from dominant but not subordinate 
      follicles. We suggest that signal transduction pathways involving Akt and Erk 
      1/Erk 2 may play an important role in determining the outcome of ovarian follicle 
      growth and development in sheep.
FAU - Evans, A C
AU  - Evans AC
AD  - Department of Animal Science and Production, Conway Institute for Biomolecular 
      and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland. 
      alex.evans@ucd.ie
FAU - Martin, F
AU  - Martin F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Estradiol/metabolism
MH  - Female
MH  - Functional Laterality
MH  - MAP Kinase Signaling System/physiology
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Ovarian Follicle/diagnostic imaging/enzymology/*physiology
MH  - Ovariectomy
MH  - Phosphorylation
MH  - Reference Values
MH  - Sheep
MH  - Ultrasonography
EDAT- 2000/12/21 11:00
MHDA- 2003/07/15 05:00
CRDT- 2000/12/21 11:00
PHST- 2000/12/21 11:00 [pubmed]
PHST- 2003/07/15 05:00 [medline]
PHST- 2000/12/21 11:00 [entrez]
AID - S0378432000002104 [pii]
AID - 10.1016/s0378-4320(00)00210-4 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2000 Dec 29;64(3-4):221-31. doi: 10.1016/s0378-4320(00)00210-4.