PMID- 11122152
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20061115
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 111
IP  - 3
DP  - 2000 Dec
TI  - Generation of functionally mature dendritic cells from the multipotential stem 
      cell line FDCP-mix.
PG  - 890-7
AB  - Dendritic cells (DCs) are crucial components of the immune system because of 
      their unique ability as antigen-presenting cells for the initiation of a primary 
      immune response. DCs, macrophages (Ms) and granulocytes (Gs) are believed to 
      originate from a common myeloid progenitor cell. However, little is known about 
      the molecular mechanisms leading to DC sublineage commitment. To establish a cell 
      system that allows the molecular and biochemical analysis of DC differentiation 
      and activation, we used the murine non-leukaemic, multipotential stem cell line 
      FDCP-mix. FDCP-mix cells were cultured in various amounts of GM-colony 
      stimulating factor (CSF) and interleukin (IL)-4 for up to 16 d and analysed for 
      morphology, expression of CD34, c-kit, Gr-1, Mac-1, CD40, MHC-I, MHC-II and 
      co-stimulatory molecules (CD80, CD86) using flow cytometry, and for their 
      capacity to present foreign antigen to autologous T cells. Up to d 7, the 
      majority of FDCP-mix cells consisted of cells differentiating along the G and M 
      lineage. Thereafter, the number of dendritic cells increased until d 13. 
      Differentiation along the DC lineage vs. the G and M lineage was favoured when 
      FDCP-mix cells were cultured in high concentration GM-CSF (500 U/ml) throughout 
      the culture and IL-4 from d 9 onwards. The dendritic cells generated from 
      FDCP-mix cells were large, non-adherent cells with veiled processes and expressed 
      MHC II, CD40, CD80 and CD86. After pulsing with a foreign antigen (keyhole limpet 
      haemocyanin), FDCP-mix-derived dendritic cells stimulated [(3)H]-thymidine 
      incorporation of naive T-cells in an autologous mixed lymphocyte reaction (MLR). 
      Our results show that functionally mature dendritic cells are generated from the 
      multipotential stem cell line FDCP-mix. This cell line thus provides the unique 
      possibility of establishing multipotential transgenic cell lines capable of 
      differentiation along the DC lineage. The experimental system described here 
      should prove a valuable tool for studying DC differentiation and function.
FAU - Schroeder, T
AU  - Schroeder T
AD  - Institute for Clinical Molecular Biology and Tumour Genetics, GSF, National 
      Research Centre for Environment and Health, Munich, Germany.
FAU - Lange, C
AU  - Lange C
FAU - Strehl, J
AU  - Strehl J
FAU - Just, U
AU  - Just U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Dendritic Cells/*physiology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Interleukin-4/*pharmacology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Microscopy, Phase-Contrast
MH  - Stem Cells/*drug effects
EDAT- 2000/12/21 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/21 11:00
PHST- 2000/12/21 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/21 11:00 [entrez]
AID - bjh2421 [pii]
PST - ppublish
SO  - Br J Haematol. 2000 Dec;111(3):890-7.