PMID- 11122260
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20190513
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 122
IP  - 3
DP  - 2000 Dec
TI  - Autoantibodies to early endosome antigen (EEA1) produce a staining pattern 
      resembling cytoplasmic anti-neutrophil cytoplasmic antibodies (C-ANCA).
PG  - 493-8
AB  - Autoantibodies to EEA1 have been described in patients with neurological 
      diseases, subacute cutaneous lupus and a variety of other conditions, including a 
      patient with Wegener's granulomatosis (WG). EEA1 is a hydrophilic peripheral 
      membrane protein transiently associated with the cytoplasmic face of early 
      endosomes. Antibodies to EEA1 produce a staining pattern that resembles the 
      C-ANCA pattern produced by anti-proteinase 3 (PR3) antibodies in WG sera. 
      Co-localization studies show incomplete overlap of the staining produced by 
      anti-EEA1 with anti-PR3. We showed that 0/40 unselected sera, from a cohort of WG 
      patients and antibodies to PR3, reacted with EEA1. In addition, 1/15 sera that 
      have a C-ANCA staining pattern but do not react with PR3 in an ELISA, 
      immunoprecipitated the recombinant EEA1 protein. We conclude that although 
      antibodies to EEA1 produce a staining pattern that resembles anti-PR3 and C-ANCA, 
      antibodies to EEA1 in WG are rare. However, some C-ANCA+ sera that do not react 
      with PR3 may contain EEA1 autoantibodies.
FAU - Selak, S
AU  - Selak S
AD  - Faculty of Medicine, University of Calgary, Calgary, Canada.
FAU - Woodman, R C
AU  - Woodman RC
FAU - Fritzler, M J
AU  - Fritzler MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Membrane Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (early endosome antigen 1)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.76 (Myeloblastin)
SB  - IM
MH  - Animals
MH  - Antibodies, Antineutrophil Cytoplasmic/*immunology
MH  - Autoantibodies/blood/*immunology
MH  - Autoantigens/*immunology
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Fluorescent Antibody Technique, Indirect
MH  - Granulomatosis with Polyangiitis/blood/*immunology
MH  - Humans
MH  - Immunoblotting/methods
MH  - Membrane Proteins/*immunology
MH  - Myeloblastin
MH  - Neutrophils/immunology
MH  - Precipitin Tests/methods
MH  - Rabbits
MH  - Serine Endopeptidases/immunology
MH  - Sodium Dodecyl Sulfate
MH  - Staining and Labeling
MH  - Tumor Cells, Cultured
MH  - Vesicular Transport Proteins
PMC - PMC1905789
EDAT- 2000/12/21 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/12/01
CRDT- 2000/12/21 11:00
PHST- 2000/12/21 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/21 11:00 [entrez]
PHST- 2001/12/01 00:00 [pmc-release]
AID - cei1390 [pii]
AID - 10.1046/j.1365-2249.2000.01390.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2000 Dec;122(3):493-8. doi: 10.1046/j.1365-2249.2000.01390.x.