PMID- 11122329
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20220408
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 12
IP  - 12
DP  - 2000 Dec
TI  - Endogenous BDNF is required for myelination and regeneration of injured sciatic 
      nerve in rodents.
PG  - 4171-80
AB  - Following a peripheral nerve injury, brain-derived neurotrophic factor (BDNF) and 
      the p75 neurotrophin receptor are upregulated in Schwann cells of the Wallerian 
      degenerating nerves. However, it is not known whether the endogenous BDNF is 
      critical for the functions of Schwann cells and regeneration of injured nerve. 
      Treatment with BDNF antibody was shown to retard the length of the regenerated 
      nerve from injury site by 24%. Histological and ultrastructural examination 
      showed that the number and density of myelinated axons in the distal side of the 
      lesion in the antibody-treated mice was reduced by 83%. In the BDNF 
      antibody-treated animals, there were only distorted and disorganized myelinated 
      fibres in the injured nerve where abnormal Schwann cells and phagocytes were 
      present. As a result of nerve degeneration in BDNF antibody-treated animals, 
      subcellular organelles, such as mitochondria, disappeared or were disorganized 
      and the laminal layers of the myelin sheath were loosened, separated or 
      collapsed. Our in situ hybridization revealed that BDNF mRNA was expressed in 
      Schwann cells in the distal segment of lesioned nerve and in the denervated 
      muscle fibres. These results indicate that Schwann cells and muscle fibres may 
      contribute to the sources of BDNF during regeneration and that the deprivation of 
      endogenous BDNF results in an impairment in regeneration and myelination of 
      regenerating axons. It is concluded that endogenous BDNF is required for 
      peripheral nerve regeneration and remyelination after injury.
FAU - Zhang, J Y
AU  - Zhang JY
AD  - Department of Anatomy, Hunan Medical University, Changsha, Hunan, People's 
      Republic of China.
FAU - Luo, X G
AU  - Luo XG
FAU - Xian, C J
AU  - Xian CJ
FAU - Liu, Z H
AU  - Liu ZH
FAU - Zhou, X F
AU  - Zhou XF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptor, Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/genetics/*physiology
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Muscle Denervation
MH  - Muscle, Skeletal/physiology
MH  - Myelin Sheath/physiology/ultrastructure
MH  - Nerve Crush
MH  - Nerve Fibers/*physiology/ultrastructure
MH  - Nerve Regeneration/*physiology
MH  - Pain/physiopathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Nerve Growth Factor/physiology
MH  - Schwann Cells/physiology/ultrastructure
MH  - Sciatic Nerve/injuries/*physiology/ultrastructure
MH  - Wallerian Degeneration
EDAT- 2000/12/21 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/21 11:00
PHST- 2000/12/21 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/21 11:00 [entrez]
AID - ejn1312 [pii]
PST - ppublish
SO  - Eur J Neurosci. 2000 Dec;12(12):4171-80.