PMID- 11122447
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 101
IP  - 4
DP  - 2000 Dec
TI  - Role of CD4(+) T helper 2-type cells in cutaneous inflammatory responses induced 
      by fluorescein isothiocyanate.
PG  - 442-51
AB  - Owing to its skin-sensitizing and fluorochromatic properties, fluorescein 
      isothiocyanate (FITC) is employed frequently as an experimental hapten in 
      mechanistic studies of contact allergy, particularly in the context of the role 
      of migration and activation of Langerhans' cells. In this study we demonstrated 
      that topical exposure of mice to FITC results in the selective development of 
      activated lymph node cells (LNC) expressing a preferential type 2 
      cytokine-secretion profile, with high levels of interleukin (IL)-4 and IL-10, but 
      low levels of interferon-gamma (IFN-gamma). Negative selection (complement 
      depletion) identified CD4(+) T helper (Th)2-type cells as the primary source in 
      activated LNC of the type 2 cytokines IL-4 and IL-10, whereas the low levels of 
      IFN-gamma produced were derived exclusively from CD8(+) T cytotoxic (Tc) 1-type 
      cells. A biphasic pattern of cutaneous inflammatory reactions was elicited by 
      exposure to FITC, the early phase of which could be transferred passively with 
      serum (presumably immunoglobulin E [IgE] antibody), whereas adoptive transfer 
      experiments demonstrated that Th2-type CD4(+) cells were responsible for the 
      delayed-type component of the dermal hypersensitivity reaction. In contrast with 
      contact allergic reactions induced by other sensitizing haptens, which are 
      considered to be largely Th1/Tc1-mediated immune processes regulated by Th2-type 
      cells, these results suggest therefore that the skin lesions provoked in mice by 
      FITC are primarily a result of the activation of Th2-type cells.
FAU - Dearman, R J
AU  - Dearman RJ
AD  - Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, UK.
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Cytokines)
RN  - 0 (Haptens)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Culture Techniques
MH  - Cytokines/biosynthesis
MH  - Dermatitis, Allergic Contact/etiology/*immunology
MH  - Female
MH  - *Fluorescein-5-isothiocyanate
MH  - Haptens/*immunology
MH  - Immunity, Cellular
MH  - Immunoglobulin E/biosynthesis
MH  - Lymph Nodes/immunology
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Th2 Cells/*immunology
PMC - PMC2327104
EDAT- 2000/12/21 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/12/01
CRDT- 2000/12/21 11:00
PHST- 2000/12/21 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/21 11:00 [entrez]
PHST- 2001/12/01 00:00 [pmc-release]
AID - 1126 [pii]
AID - 10.1046/j.1365-2567.2000.01126.x [doi]
PST - ppublish
SO  - Immunology. 2000 Dec;101(4):442-51. doi: 10.1046/j.1365-2567.2000.01126.x.