PMID- 1112261
OWN - NLM
STAT- MEDLINE
DCOM- 19750506
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 96
IP  - 2
DP  - 1975 Feb
TI  - Formation and secretion of 5alpha-androstan-17beta-ol-3-one, 
      5alpha-androstan-3alpha,17beta-diol and 5alpha-androstan-3beta,17beta-diol by the 
      perfused rabbit testis epididymis.
PG  - 479-85
AB  - Rabbit testes epididymides were perfused in vitro at 36.5 C with an artificial 
      medium containing bovine red blooc cells. Three major testosterone metabolites 
      were formed and subsequently elaborated into the venous effluent of testes 
      epididymibdes when testosterone-1,2,6,7-3-H was infused into the artery of the 
      perfused organ. In contrast, testosterone-1,2,6,7-1-H remained unchanged when 
      incubated in the artificial medium under similar conditions. Elution volume from 
      a Sephadex LH-20 column, development in three ascending paper chromatographic 
      (APC) systems, acetylation and development in a fourth APC system followed by gas 
      liquid chromatography (GLC) of the acetylated products tentatively identified the 
      radioactive-metabolites as 5alpha-androstan-17beta-ol-3-one (DHT), 
      5alpha-androstan-3alpha,17beta-diol (3alphaol) and 
      5alpha-androstan-3beta,17beta-diol (3beta-ol). The identity of these 
      5alpha-reduced testosterone metabolites was confirmed by crystallization to 
      constant specific activity in the presence of authentic carrier steroid. These 
      results suggest that DHT and androstanediols formed by perfused rabbit testes 
      epididymides subsequently escape into the venous effluent.
FAU - Ewing, L
AU  - Ewing L
FAU - Brown, B
AU  - Brown B
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Androstanes)
RN  - 0 (Hydroxysteroids)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Androstanes/*metabolism
MH  - Animals
MH  - Chromatography, Gas
MH  - Chromatography, Paper
MH  - Dihydrotestosterone/*metabolism
MH  - Epididymis/*metabolism
MH  - Hydroxysteroids/*metabolism
MH  - Male
MH  - Oxidation-Reduction
MH  - Perfusion
MH  - Rabbits
MH  - Testosterone/*metabolism
EDAT- 1975/02/01 00:00
MHDA- 1975/02/01 00:01
CRDT- 1975/02/01 00:00
PHST- 1975/02/01 00:00 [pubmed]
PHST- 1975/02/01 00:01 [medline]
PHST- 1975/02/01 00:00 [entrez]
AID - 10.1210/endo-96-2-479 [doi]
PST - ppublish
SO  - Endocrinology. 1975 Feb;96(2):479-85. doi: 10.1210/endo-96-2-479.