PMID- 11124540
OWN - NLM
STAT- MEDLINE
DCOM- 20010201
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 90
IP  - 3-4
DP  - 2000
TI  - Localization by FISH of the 31 Texas nomenclature type I markers to both Q- and 
      R-banded bovine chromosomes.
PG  - 315-20
AB  - A series of 31 marker genes (one per chromosome) were localized precisely to both 
      Q- and R-banded bovine chromosomes by fluorescence in situ hybridization (FISH), 
      as a contribution to the revised chromosome nomenclature of the three major 
      domestic bovidae (cattle, sheep and goat). All marker genes except one (LDHA) are 
      taken from the Texas Nomenclature of the cattle karyotype published in 1996. 
      Homologous probes for each marker gene were obtained by screening a bovine BAC 
      library by PCR with specific primer pairs. After labeling with biotin, each probe 
      preparation was divided into two fractions and hybridized to bovine chromosomes 
      identified either by Q or R banding. Clear signals and good quality band patterns 
      were observed in all cases. Results of the two series of hybridizations are 
      totally concordant both for Q and R band chromosome numbering and precise band 
      localization. This work permits an unambiguous correlation between the Q/G- and 
      R-banded 31 bovine chromosomes, including chromosomes 25, 27 and 29 which 
      remained unresolved in the Texas Nomenclature (1996). Hybridization of the 
      chromosome 29 marker gene to metaphase spreads from a 1;29 Robertsonian 
      translocation bull carrier showed a positive signal on the short arm of this 
      rearranged chromosome, confirming that the numbering of this long-known 
      translocation in cattle is correct when referring to the Texas Nomenclature 
      (1996). Taking into account that cattle, goat and sheep have very similar banded 
      karyotypes, the data presented here will help to establish a definite and 
      complete reference chromosome nomenclature for these species.
CI  - Copyright 2000 S. Karger AG, Basel.
FAU - Hayes, H
AU  - Hayes H
AD  - Institut National de la Recherche Agronomique, INRA-CRJ, Jouy-en-Josas, France. 
      hayes@biotec.jouy.inra.fr
FAU - Di Meo, G P
AU  - Di Meo GP
FAU - Gautier, M
AU  - Gautier M
FAU - Laurent, P
AU  - Laurent P
FAU - Eggen, A
AU  - Eggen A
FAU - Iannuzzi, L
AU  - Iannuzzi L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Biotinylation
MH  - Cattle
MH  - *Chromosome Banding/standards
MH  - Chromosomes/genetics
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - DNA Probes
MH  - Genetic Markers/*genetics
MH  - Goats/*genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Physical Chromosome Mapping/standards
MH  - Sheep/*genetics
MH  - *Terminology as Topic
MH  - Texas
EDAT- 2000/12/22 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/22 11:00
PHST- 2000/12/22 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/22 11:00 [entrez]
AID - 56795 [pii]
AID - 10.1159/000056795 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2000;90(3-4):315-20. doi: 10.1159/000056795.