PMID- 11125158
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20231213
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 82
IP  - Pt 1
DP  - 2001 Jan
TI  - Complete nucleotide sequence and host range of South African cassava mosaic 
      virus: further evidence for recombination amongst begomoviruses.
PG  - 53-58
LID - 10.1099/0022-1317-82-1-53 [doi]
AB  - Complete nucleotide sequences of the DNA-A (2800 nt) and DNA-B (2760 nt) 
      components of a novel cassava-infecting begomovirus, South African cassava mosaic 
      virus (SACMV), were determined and compared with various New World and Old World 
      begomoviruses. SACMV is most closely related to East African cassava mosaic virus 
      (EACMV) in both its DNA-A (85% with EACMV-MH and -MK) and -B (90% with 
      EACMV-UG2-Mld and EACMV-UG3-Svr) components; however, percentage sequence 
      similarities of less than 90% in the DNA-A component allowed SACMV to be 
      considered a distinct virus. One significant recombination event spanning the 
      entire AC4 open reading frame was identified; however, there was no evidence of 
      recombination in the DNA-B component. Infectivity of the cloned SACMV genome was 
      demonstrated by successful agroinoculation of cassava and three other plant 
      species (Phaseolus vulgaris, Malva parviflora and Nicotiana benthamiana). This is 
      the first description of successful infection of cassava with a geminivirus using 
      Agrobacterium tumefaciens.
FAU - Berrie, L C
AU  - Berrie LC
AD  - Department of Molecular and Cell Biology, University of the Witwatersrand, 
      Private Bag 3, PO Wits 2050, Johannesburg, South Africa1.
FAU - Rybicki, E P
AU  - Rybicki EP
AD  - Department of Microbiology, University of Cape Town, Private Bag, Rondebosch 
      7701, Cape Town, South Africa2.
FAU - Rey, M E C
AU  - Rey MEC
AD  - Department of Molecular and Cell Biology, University of the Witwatersrand, 
      Private Bag 3, PO Wits 2050, Johannesburg, South Africa1.
LA  - eng
SI  - GENBANK/AF155806
SI  - GENBANK/AF155807
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Agrobacterium tumefaciens/genetics
MH  - Cloning, Molecular
MH  - Fabaceae/virology
MH  - Geminiviridae/classification/*genetics
MH  - *Genome, Viral
MH  - Malvaceae/virology
MH  - Manihot/*virology
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Plants, Medicinal
MH  - Plants, Toxic
MH  - Recombination, Genetic
MH  - Nicotiana/virology
MH  - Transformation, Genetic
EDAT- 2000/12/23 11:00
MHDA- 2001/03/07 10:01
CRDT- 2000/12/23 11:00
PHST- 2000/12/23 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2000/12/23 11:00 [entrez]
AID - 10.1099/0022-1317-82-1-53 [doi]
PST - ppublish
SO  - J Gen Virol. 2001 Jan;82(Pt 1):53-58. doi: 10.1099/0022-1317-82-1-53.