PMID- 11125306
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20061115
IS  - 1148-5493 (Print)
IS  - 1148-5493 (Linking)
VI  - 11
IP  - 4
DP  - 2000 Dec
TI  - Regulation of interleukin-18 (IL-18) expression in keratinocytes (HaCaT): 
      implications for early wound healing.
PG  - 626-33
AB  - Keratinocytes display a high basal level expression of IL-18. Tumor necrosis 
      factor-alpha (TNF-alpha) mediated a large decrease in IL-18 mRNA levels in the 
      human keratinocyte cell line HaCaT, which was accompanied by a subsequent 
      accumulation of IL-18 protein in the cell culture supernatants, which was shown 
      to be biologically active. By contrast, epidermal growth factor (EGF) and 
      transforming growth factor-alpha (TGF-alpha), respectively, strongly decreased 
      IL-18 mRNA expression in HaCaT keratinocytes in the absence of IL-18 protein 
      release from the cells. Notably, a pre-treatment of the cells with EGF, or 
      TGF-alpha clearly attenuated TNF-alpha-induced IL-18 protein, release and 
      bioactivity. For the in vivo situation of cutaneous wound repair, we observed an 
      increase in IL-18 protein, 10 hours post-wounding, that closely correlated to 
      infiltration of neutrophils which are known as producers of TNF-alpha. Our data 
      suggest that bioactive IL-18 might be tightly counter-regulated by platelet- and 
      neutrophil-derived factors at the onset of repair.
FAU - Kampfer, H
AU  - Kampfer H
AD  - Zentrum der Pharmakologie, Klinikum der Johann Wolfgang goethe-Universitat, 
      Frankfurt am Main, Germany.
FAU - Muhl, H
AU  - Muhl H
FAU - Manderscheid, M
AU  - Manderscheid M
FAU - Kalina, U
AU  - Kalina U
FAU - Kauschat, D
AU  - Kauschat D
FAU - Pfeilschifter, J
AU  - Pfeilschifter J
FAU - Frank, S
AU  - Frank S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur Cytokine Netw
JT  - European cytokine network
JID - 9100879
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Blood Platelets/immunology
MH  - Cell Line
MH  - Epidermal Growth Factor/*immunology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-18/*genetics/immunology
MH  - Keratinocytes/drug effects/*immunology
MH  - Neutrophil Infiltration/immunology
MH  - Neutrophils/immunology
MH  - RNA, Messenger/analysis
MH  - Transforming Growth Factor alpha/*immunology
MH  - Tumor Necrosis Factor-alpha/*immunology
MH  - Wound Healing/*immunology
EDAT- 2000/12/23 11:00
MHDA- 2001/03/10 10:01
CRDT- 2000/12/23 11:00
PHST- 2000/12/23 11:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2000/12/23 11:00 [entrez]
PST - ppublish
SO  - Eur Cytokine Netw. 2000 Dec;11(4):626-33.