PMID- 11125539
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20131121
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 14
IP  - 5
DP  - 2000 Sep-Oct
TI  - Expression of matrix metalloproteinases and their inhibitors by rat NK cells: 
      inhibition of their expression by genistein.
PG  - 557-64
AB  - In this study, we describe rat NK cell-derived MMPs including membrane-type MMPs 
      (MT-MMPs) and tissue inhibitors of MMP (TIMPs). RT-PCR analysis from cDNA of rat 
      A-NK cells revealed mRNA for MMP-2, MMP-9, MMP-7, MMP-10, MMP-11, MMP-13, 
      MT1-MMP, MT2-MMP, TIMP-1, and TIMP-2. The RNK-16 cells expressed mRNA for MMP-7, 
      MMP-10, MMP-11, MT1-MMP, MT2-MMP, TIMP-1, and TIMP-2, in addition to MMP-3 and 
      MMP-13. Western blot analysis confirmed proteins for MT1-MMP and MT2-MMP in 
      RNK-16 cells. TIMP-1 in rat A-NK cells was present at molecular mass of 34-kDa 
      protein which may represent a highly glycosylated form. Genistein, a natural 
      isoflavone found in soybeans, inhibited proliferation of RNK-16 cells in dosage 
      dependent manner. In addition, it down-regulated the expression of MMP-13, 
      MT1-MMP, TIMP-1 and TIMP-2. Moreover, genistein greatly impaired the ability of 
      RNK-16 cells to invade through a model basement membrane. This effect might be 
      mediated by the observed down-regulation of MMP-13 and MT1-MMP.
FAU - Kim, M H
AU  - Kim MH
AD  - Department of Molecular Biology and Immunology, and Institute for Cancer 
      Research, University of North Texas Health Science Center at Fort Worth, Texas, 
      USA
FAU - Albertsson, P
AU  - Albertsson P
FAU - Xue, Y
AU  - Xue Y
FAU - Kitson, R P
AU  - Kitson RP
FAU - Nannmark, U
AU  - Nannmark U
FAU - Goldfarb, R H
AU  - Goldfarb RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - DH2M523P0H (Genistein)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Division/drug effects
MH  - DNA Primers/chemistry
MH  - DNA, Complementary/analysis
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation
MH  - Enzyme Inhibitors/*pharmacology
MH  - Genistein/*pharmacology
MH  - Killer Cells, Natural/drug effects/*enzymology
MH  - Male
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinases/genetics/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 2000/12/28 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/28 11:00
PHST- 2000/12/28 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/28 11:00 [entrez]
PST - ppublish
SO  - In Vivo. 2000 Sep-Oct;14(5):557-64.