PMID- 11126124
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20180928
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 29
IP  - 6
DP  - 2000 Dec
TI  - The use of fluorescence in situ hybridization (FISH) on paraffin-embedded tissue 
      sections for the study of microchimerism.
PG  - 1220-4
AB  - We describe here a simple and versatile method of fluorescence in situ 
      hybridization (FISH) on paraffin-embedded tissue sections with specific 
      application in the study of microchimerism, that is, the presence of intact 
      foreign cells within an individual. This is accomplished through the use of X and 
      Y chromosome-specific probes to identify the presence of male nuclei within a 
      tissue section from a female, and vice versa. This technique requires only minor 
      modification if at first the hybridization does not yield fluorescent signals of 
      high quality. Analysis of a wide variety of tissue types is possible with this 
      method, and multiple tissue types from one or more individuals can be processed 
      in the same hybridization reaction. This robust FISH method has been used 
      successfully in our laboratory to investigate fetal cell microchimerism in the 
      following paraffin-embedded tissue types: skin, lung, thyroid, adrenal gland, 
      lymph node, heart, spleen, liver, pancreas, kidney, and intestine.
FAU - Johnson, K L
AU  - Johnson KL
AD  - New England Medical Center, Tufts University School of Medicine, Boston, MA, USA.
FAU - Zhen, D K
AU  - Zhen DK
FAU - Bianchi, D W
AU  - Bianchi DW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
SB  - IM
MH  - Cell Nucleus/genetics
MH  - Chimera/*genetics
MH  - Female
MH  - Fetus
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Maternal-Fetal Exchange/genetics
MH  - Organ Specificity/genetics
MH  - *Paraffin Embedding
MH  - Pregnancy
MH  - X Chromosome/chemistry
MH  - Y Chromosome/chemistry
EDAT- 2000/12/29 11:00
MHDA- 2001/04/06 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - 10.2144/00296st01 [doi]
PST - ppublish
SO  - Biotechniques. 2000 Dec;29(6):1220-4. doi: 10.2144/00296st01.