PMID- 11127395
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20191025
IS  - 0888-0018 (Print)
IS  - 0888-0018 (Linking)
VI  - 17
IP  - 8
DP  - 2000 Dec
TI  - Childhood all blasts retain phenotypic and genotypic characteristics upon 
      long-term serial passage in NOD/SCID mice.
PG  - 635-50
AB  - For children with an early bone marrow relapse or relapsed T-cell acute 
      lymphoblastic leukemia (ALL), allogeneic bone marrow transplantation (BMT) is 
      currently the only therapeutic option with a curative approach. Here, the graft 
      versus leukemia (GvL) effect seems to play an important role for long-term 
      immunological control of leukemia. If a bone marrow donor is not available, 
      autologous stem cell transplantation after high-dose chemotherapy has been used 
      as an alternative option. The objective of this work was the induction of tumor 
      specific cytotoxic T-lymphocytes (CTL) against autologous leukemic cells in order 
      to generate the missing GvL effect after autoBMT. The first step was the 
      establishment of an optimized and reliable mouse model. The second step was the 
      induction of a GvL effect in an allogeneic approach to serve as a basis for 
      further GvL experiments in an autologous approach in this mouse model. Leukemic 
      cells from 11 out of 16 different pediatric patients were successfully 
      established in mice and in one case passaged over 19 generations without changes 
      of genotype or phenotype. The antileukemic activity of allogeneic human MNC as a 
      GvL reaction and an accompanying GvHD in the mouse model was shown. 
      Xenotransplanted ALL can be considered a clinically relevant model mimicking the 
      human conditions and as a useful preclinical tool for the evaluation of novel 
      immuno- or genetherapeutic approaches.
FAU - Borgmann, A
AU  - Borgmann A
AD  - Department of Pediatric Oncology/Hematology, Charite, Campus Virchow-Klinikum, 
      Berlin, Germany.
FAU - Baldy, C
AU  - Baldy C
FAU - von Stackelberg, A
AU  - von Stackelberg A
FAU - Beyermann, B
AU  - Beyermann B
FAU - Fichtner, I
AU  - Fichtner I
FAU - Nurnberg, P
AU  - Nurnberg P
FAU - Henze, G
AU  - Henze G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pediatr Hematol Oncol
JT  - Pediatric hematology and oncology
JID - 8700164
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Adolescent
MH  - Animals
MH  - Cell Division
MH  - Child
MH  - Child, Preschool
MH  - DNA Fingerprinting
MH  - Female
MH  - Genotype
MH  - Graft vs Host Disease/immunology/pathology
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-2/pharmacology
MH  - Leukocytes, Mononuclear/drug effects/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Neoplasm Transplantation/*methods
MH  - Phenotype
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/*immunology/pathology
MH  - T-Lymphocytes, Cytotoxic/drug effects/immunology
MH  - Transplantation, Heterologous
EDAT- 2000/12/29 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - 10.1080/08880010050211349 [doi]
PST - ppublish
SO  - Pediatr Hematol Oncol. 2000 Dec;17(8):635-50. doi: 10.1080/08880010050211349.