PMID- 11129444
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20191104
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 9
IP  - 4
DP  - 2000 Dec
TI  - Comparison of a multiplex reverse transcriptase-polymerase chain reaction for 
      BCR-ABL to fluorescence in situ hybridization, Southern blotting, and 
      conventional cytogenetics in the monitoring of patients with Ph1-positive 
      leukemias.
PG  - 203-9
AB  - A multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) assay for 
      both major forms of BCR-ABL was compared with fluorescence in situ hybridization 
      (FISH), karyotyping, and Southern blotting for disease monitoring in 37 follow-up 
      bone marrow samples from 32 patients with Ph1-positive leukemia. Of these 37 
      samples, 33 were from patients with chronic myeloid leukemia (CML) (26 post 
      allogeneic bone marrow transplantation [AlloBMT] and seven during 
      interferon-alpha therapy) and 4 from Ph1-positive acute lymphoblastic leukemia 
      (ALL) patients (1 post AlloBMT and 3 post high dose chemotherapy). For the 27 
      samples studied after AlloBMT (26 CML and 1 Ph1-positive ALL) the time after 
      transplantation ranged from 1 to 107 months (median 47.5 months). In 8 (22%) of 
      the 37 samples there were discrepant results among methods. The discrepancy rates 
      relative to other techniques were: karyotyping 17% (5 of 29), Southern blotting 
      18% (6 of 33), multiplex RT-PCR 8% (3 of 37), and FISH 8% (3 of 37). Therefore, 
      the relative accuracy of each method for disease monitoring in Ph1-positive 
      leukemia was: 83% (24 of 29) for karyotyping, 82% (27 of 33) for Southern 
      blotting, 92% (34 of 37) for FISH, and 92% (34 of 37) for multiplex RT-PCR. This 
      multiplex RT-PCR assay appears equivalent to FISH in terms of accuracy, 
      simplicity, and turnaround time and both are superior to Southern blot and 
      conventional cytogenetics in the laboratory monitoring of Ph1-positive leukemias.
FAU - Colleoni, G W
AU  - Colleoni GW
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New 
      York 10021, USA.
FAU - Jhanwar, S C
AU  - Jhanwar SC
FAU - Ladanyi, M
AU  - Ladanyi M
FAU - Chen, B
AU  - Chen B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Blotting, Southern
MH  - Child
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Genes, abl/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - K562 Cells
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2000/12/29 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - 10.1097/00019606-200012000-00005 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2000 Dec;9(4):203-9. doi: 10.1097/00019606-200012000-00005.