PMID- 11129499
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20190906
IS  - 0901-9928 (Print)
IS  - 0901-9928 (Linking)
VI  - 87
IP  - 5
DP  - 2000 Nov
TI  - Impairment of the Ca2+-permeable AMPA/kainate receptors by lead exposure in 
      organotypic rat hippocampal slice cultures.
PG  - 204-10
AB  - Previous studies have demonstrated that chronic lead exposure may impair neuronal 
      process underlying synaptic plasticity via a direct interaction with 
      N-methyl-D-aspartate (NMDA) receptors. The present study was carried out to 
      investigate the effects of lead exposure on non-NMDA 
      (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid, AMPA/kainate) 
      receptors of rat hippocampus. Ca2+-permeable AMPA/kainate receptors in 
      organotypic slice cultures were evaluated by using cobalt uptake, a histochemical 
      method that identifies cells expressing Ca2+-permeable non-NMDA receptors. Ten mM 
      L-glutamate-induced cobalt accumulation was enriched in area CA1, area CA3 and in 
      dentate gyrus, which was totally blocked by 100 microM 
      DL-2-amino-5-phosphonovaleric acid (AP5) and 100 microM 
      6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX). Three hundred microM NMDA-induced 
      cobalt accumulation was in area CA1, area dentate gyrus and was blocked by AP5 or 
      CNQX. One hundred microM AMPA had effects in area CA1, area CA3 and in dentate 
      gyrus, which were blocked by CNQX, not by AP5. Furthermore, cobalt accumulations 
      induced by NMDA and AMPA in the lead-exposed rats decreased significantly than 
      those in the controls. The results indicate that AMPA receptors enriched in area 
      CA1, area CA3, area dentate gyrus and kainate receptors enriched in area CA1, 
      area dentate gyrus are impaired by lead exposure.
FAU - Sui, L
AU  - Sui L
AD  - School of Life Science, University of Science and Technology of China, Hefei, 
      Anhui, People's Republic of China.
FAU - Ruan, D Y
AU  - Ruan DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Pharmacol Toxicol
JT  - Pharmacology & toxicology
JID - 8702180
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Kainic Acid)
RN  - 2P299V784P (Lead)
RN  - 3G0H8C9362 (Cobalt)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/pharmacology
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Animals, Suckling
MH  - Biological Transport/drug effects
MH  - Calcium/*metabolism
MH  - Cobalt/analysis/metabolism
MH  - Glutamic Acid/pharmacology
MH  - Hippocampus/*drug effects/metabolism
MH  - Lead/analysis/*toxicity
MH  - N-Methylaspartate/pharmacology
MH  - Organ Culture Techniques
MH  - Permeability
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, AMPA/*metabolism
MH  - Receptors, Kainic Acid/*metabolism
EDAT- 2000/12/29 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - 10.1034/j.1600-0773.2000.d01-75.x [doi]
PST - ppublish
SO  - Pharmacol Toxicol. 2000 Nov;87(5):204-10. doi: 10.1034/j.1600-0773.2000.d01-75.x.