PMID- 11129609
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20131121
IS  - 0916-8451 (Print)
IS  - 0916-8451 (Linking)
VI  - 64
IP  - 10
DP  - 2000 Oct
TI  - Phosphorylation of guanosine using guanosine-inosine kinase from Exiguobacterium 
      acetylicum coupled with ATP regeneration.
PG  - 2259-61
AB  - Guanosine 5'-monophosphate (5'-GMP) and inosine 5'-monophosphate (5'-IMP) are 
      widely used as flavor enhancers. Recently, a novel process for 5'-IMP production 
      by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP 
      regeneration was reported. In this study, we demonstrated the practical 
      possibility of producing 5'-GMP by phosphorylation of guanosine using a 
      guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP 
      regeneration.
FAU - Kawasaki, H
AU  - Kawasaki H
AD  - Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, 
      Ajinomoto Co. Inc., Kawasaki-shi, Japan. hisashi_kawasaki@ajinomoto.com
FAU - Usuda, Y
AU  - Usuda Y
FAU - Shimaoka, M
AU  - Shimaoka M
FAU - Utagawa, T
AU  - Utagawa T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 12133JR80S (Guanosine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.73 (inosine kinase)
SB  - IM
MH  - Adenosine Triphosphate/*metabolism
MH  - Bacillus/*enzymology
MH  - Catalysis
MH  - Guanosine/*metabolism
MH  - Phosphorylation
MH  - Phosphotransferases (Alcohol Group Acceptor)/*metabolism
MH  - Staphylococcus/*enzymology
EDAT- 2000/12/29 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - 10.1271/bbb.64.2259 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2000 Oct;64(10):2259-61. doi: 10.1271/bbb.64.2259.