PMID- 11131334
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20190822
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 54
IP  - 7
DP  - 2000 Oct 15
TI  - Measurement of immunoglobulin G, A and M concentrations in boar seminal plasma.
PG  - 1171-84
AB  - How the immune system relates to the boar reproductive tract is not well defined. 
      This is an important area of study because disease-causing agents may be 
      transmitted through boar semen. We have previously identified porcine 
      reproductive and respiratory syndrome virus (PRRSV) in boar semen and wanted to 
      identify PRRSV-specific antibodies within seminal plasma. However, literature 
      documenting total immunoglobulin concentration or the predominant immunoglobulin 
      isotype in boar semen was not available. Therefore, we developed a sandwich 
      enzyme-linked immunoassay (ELISA) to quantitate total IgG, IgA and IgM in seminal 
      plasma from 16 healthy, nonvaccinated, adult boars (n = 102 semen samples). In 
      seminal plasma, IgG was the predominant isotype followed by IgA and IgM. Mean 
      levels +/- the standard deviation followed by the 95% confidence interval of IgG, 
      IgA and IgM were 23.2 +/- 14 microg/mL (15.5 to 31.0), 4.8 +/- 2.5 microg/mL (3.5 
      to 6.2) and 3.7 +/- 1.7 microg/mL (2.7 to 4.7), respectively. These 
      concentrations of immunoglobulins in seminal plasma were considerably lower than 
      in other swine secretions, which might allow for the survival of infectious 
      agents in boar semen.
FAU - Kaiser, T J
AU  - Kaiser TJ
AD  - Animal Disease Research and Diagnostic Laboratory, South Dakota State University, 
      Brookings 57007, USA.
FAU - Christopher-Hennings, J
AU  - Christopher-Hennings J
FAU - Nelson, E A
AU  - Nelson EA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoglobulin A/*analysis
MH  - Immunoglobulin G/*analysis
MH  - Immunoglobulin M/*analysis
MH  - Male
MH  - Reference Values
MH  - Semen/*immunology
MH  - Swine/*immunology
EDAT- 2000/12/29 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - S0093-691X(00)00424-6 [pii]
AID - 10.1016/s0093-691x(00)00424-6 [doi]
PST - ppublish
SO  - Theriogenology. 2000 Oct 15;54(7):1171-84. doi: 10.1016/s0093-691x(00)00424-6.