PMID- 11131921
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20190921
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 79
IP  - 11
DP  - 2000 Nov
TI  - Early detection of relapse and evaluation of treatment for mixed chimerism using 
      fluorescence in situ hybridization following allogeneic hematopoietic cell 
      transplant for hematological malignancies.
PG  - 622-6
AB  - In order to detect chimerism, fluorescence in situ hybridization (FISH) and 
      cytogenetic analyses were performed on bone marrow cells from 47 patients with 
      hematological malignancies following allogeneic hematopoietic cell transplant 
      (HCT). The dual-color XY, major Bcr-Abl (M-Bcr-Abl), and specific alpha-satellite 
      probes were used for sex-mismatched HCT, chronic myeloid leukemia (CML), and 
      myelodysplastic syndrome (MDS) cases with karyotypic abnormalities before HCT, 
      respectively. Donor cells were found using FISH analysis in all 32 cases examined 
      within 2 months following HCT, confirming engraftment. In six cases, however, 
      cytogenetic analysis failed to detect donor cells due to lack of metaphases. 
      Relapse occurred in four of the six cases in which mixed chimerism was detected 
      using FISH analysis after 6 months of HCT. In contrast, after 12 months of HCT, 
      no relapse was found in 24 patients without host cells. For two patients with 
      mixed chimerism, gradual reduction of immunosuppressants or donor lymphocyte 
      infusion resulted in the disappearance of host cells as analyzed using FISH 
      analysis. In three extramedullary relapse cases, however, cytogenetic relapse 
      preceded morphological and FISH relapse. These findings suggest that FISH 
      analysis is more useful for detecting residual host cells after HCT, and the 
      combination of FISH and cytogenetic analyses provide a more detailed evaluation 
      for HCT patients. The results also indicate that monitoring of mixed chimerism 
      using FISH analysis after 6 months of HCT is important for allowing the early 
      detection of hematological relapse.
FAU - Tamura, S
AU  - Tamura S
AD  - Second Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, 
      Japan. samtam@med.email.ne.jp
FAU - Saheki, K
AU  - Saheki K
FAU - Takatsuka, H
AU  - Takatsuka H
FAU - Wada, H
AU  - Wada H
FAU - Fujimori, Y
AU  - Fujimori Y
FAU - Okamoto, T
AU  - Okamoto T
FAU - Takemoto, Y
AU  - Takemoto Y
FAU - Hashimoto-Tamaoki, T
AU  - Hashimoto-Tamaoki T
FAU - Furuyama, J
AU  - Furuyama J
FAU - Kakishita, E
AU  - Kakishita E
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
SB  - IM
MH  - Female
MH  - Graft vs Leukemia Effect/drug effects
MH  - Hematologic Neoplasms/diagnosis/*therapy
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Recurrence
MH  - *Transplantation Chimera
EDAT- 2000/12/29 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/29 11:00
PHST- 2000/12/29 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/29 11:00 [entrez]
AID - 10.1007/s002770000204 [doi]
PST - ppublish
SO  - Ann Hematol. 2000 Nov;79(11):622-6. doi: 10.1007/s002770000204.