PMID- 11133005
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 67
IP  - 24
DP  - 2000 Nov 3
TI  - Hypoxic conditions restore lost sensitivity to the growth inhibitory effect of 
      transforming growth factor beta-1 (TGF beta-1) in proliferating rat hepatocytes 
      in vitro.
PG  - 2929-40
AB  - TGF beta-1 is known to be a growth inhibitor of regenerating liver, and an 
      inducer of hepatocyte apoptosis in primary culture. However, hepatocytes can 
      proliferate after partial hepatectomy even at high serum TGF beta-1 
      concentrations. In this study we used the primary cultures of rat hepatocytes for 
      10 days to investigate how TGF beta-1 affects proliferating hepatocytes. DNA 
      synthesis peaked on day 8 of culture, and TGF beta-1-induced apoptosis was 
      significantly suppressed on day 8 compared to days 2, 5, and 10. Flow-cytometric 
      analysis revealed that hepatocytes that had incorporated BrdU were resistant to 
      the apoptotic effect of TGF beta-1, and Northern blot analysis showed that TGF 
      beta receptor mRNA was down-regulated on day 8. Hypoxic conditions restores TGF 
      beta receptor mRNA expression and the lost sensitivity of proliferating 
      hepatocyte to TGF beta-1.
FAU - Kakihara, N
AU  - Kakihara N
AD  - The First Department of Surgery, Shiga University of Medical Science 
      Seta-tsukinowacho, Otsu-city, Japan. kakihara@belle.shiga-med.ac.jp
FAU - Takeshita, K
AU  - Takeshita K
FAU - Ishibashi, H
AU  - Ishibashi H
FAU - Tani, T
AU  - Tani T
FAU - Kodama, M
AU  - Kodama M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-49-2 (DNA)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Blotting, Northern
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Division
MH  - Cell Hypoxia/drug effects
MH  - Cells, Cultured
MH  - DNA/biosynthesis/drug effects
MH  - Down-Regulation
MH  - Flow Cytometry
MH  - Hepatocytes/cytology/*drug effects
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Receptors, Transforming Growth Factor beta/genetics/metabolism
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 2001/01/02 11:00
MHDA- 2001/02/28 10:01
CRDT- 2001/01/02 11:00
PHST- 2001/01/02 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/01/02 11:00 [entrez]
AID - S002432050000878X [pii]
AID - 10.1016/s0024-3205(00)00878-x [doi]
PST - ppublish
SO  - Life Sci. 2000 Nov 3;67(24):2929-40. doi: 10.1016/s0024-3205(00)00878-x.