PMID- 11133434
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 67
IP  - 1
DP  - 2001 Jan
TI  - Characterization of a novel spirochete associated with the hydrothermal vent 
      polychaete annelid, Alvinella pompejana.
PG  - 110-7
AB  - A highly integrated, morphologically diverse bacterial community is associated 
      with the dorsal surface of Alvinella pompejana, a polychaetous annelid that 
      inhabits active high-temperature deep-sea hydrothermal vent sites along the East 
      Pacific Rise (EPR). Analysis of a previously prepared bacterial 16S ribosomal DNA 
      (rDNA) library identified a spirochete most closely related to an endosymbiont of 
      the oligochete Olavius loisae. This spirochete phylotype (spirochete A) comprised 
      only 2.2% of the 16S rDNA clone library but appeared to be much more dominant 
      when the same sample was analyzed by denaturing gradient gel electrophoresis 
      (DGGE) and the terminal restriction fragment length polymorphism procedure (12 to 
      18%). PCR amplification of the community with spirochete-specific primers used in 
      conjunction with DGGE analysis identified two spirochete phylotypes. The first 
      spirochete was identical to spirochete A but was present in only one A. pompejana 
      specimen. The second spirochete (spirochete B) was 84.5% similar to spirochete A 
      and, more interestingly, was present in the epibiont communities of all of the A. 
      pompejana specimens sampled throughout the geographic range of the worm (13 
      degrees N to 32 degrees S along the EPR). The sequence variation of the 
      spirochete B phylotype was less than 3% for the range of A. pompejana specimens 
      tested, suggesting that a single spirochete species was present in the A. 
      pompejana epibiotic community. Additional analysis of the environments 
      surrounding the worm revealed that spirochetes are a ubiquitous component of 
      high-temperature vents and may play an important role in this unique ecosystem.
FAU - Campbell, B J
AU  - Campbell BJ
AD  - University of Delaware Graduate College of Marine Studies, Lewes, Delaware 19958, 
      USA.
FAU - Cary, S C
AU  - Cary SC
LA  - eng
SI  - GENBANK/AF180309
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - DNA, Bacterial/analysis/genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - Electrophoresis/methods
MH  - *Marine Biology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polychaeta/*microbiology
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Spirochaeta/*classification/genetics/physiology
MH  - Symbiosis
MH  - Temperature
PMC - PMC92526
EDAT- 2001/01/03 11:00
MHDA- 2001/05/18 10:01
PMCR- 2001/01/01
CRDT- 2001/01/03 11:00
PHST- 2001/01/03 11:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/01/03 11:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 1124 [pii]
AID - 10.1128/AEM.67.1.110-117.2001 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2001 Jan;67(1):110-7. doi: 10.1128/AEM.67.1.110-117.2001.