PMID- 11134269
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20181130
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 75
IP  - 2
DP  - 2001 Jan
TI  - Modulation of immunity against herpes simplex virus infection via mucosal genetic 
      transfer of plasmid DNA encoding chemokines.
PG  - 569-78
AB  - In this study, we examined the effects of murine chemokine DNA, as genetic 
      adjuvants given mucosally, on the systemic and distal mucosal immune responses to 
      plasmid DNA encoding gB of herpes simplex virus (HSV) by using the mouse model. 
      The CC chemokines macrophage inflammatory protein 1beta (MIP-1beta) and monocyte 
      chemotactic protein 1 (MCP-1) biased the immunity to the Th2-type pattern as 
      judged by the ratio of immunoglobulin isotypes and interleukin-4 cytokine levels 
      produced by CD4(+) T cells. The CXC chemokine MIP-2 and the CC chemokine 
      MIP-1alpha, however, mounted immune responses of the Th1-type pattern, and such a 
      response rendered recipients more resistant to HSV vaginal infection. In 
      addition, MIP-1alpha appeared to act via the upregulation of antigen-presenting 
      cell (APC) function and the expression of costimulatory molecules (B7-1 and 
      B7-2), whereas MIP-2 enhanced Th1-type CD4(+) T-cell-mediated adaptive immunity 
      by increasing gamma interferon secretion from activated NK cells. Our results 
      emphasize the value of using the mucosal route to administer DNA modulators such 
      as chemokines that function as adjuvants by regulating the activity of innate 
      immunity. Our findings provide new insight into the value of CXC and CC 
      chemokines, which act on different innate cellular components as the linkage 
      signals between innate and adaptive immunity in mucosal DNA vaccination.
FAU - Eo, S K
AU  - Eo SK
AD  - Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996, 
      USA.
FAU - Lee, S
AU  - Lee S
FAU - Chun, S
AU  - Chun S
FAU - Rouse, B T
AU  - Rouse BT
LA  - eng
GR  - R01 AI046462/AI/NIAID NIH HHS/United States
GR  - AI46462/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Chemokine CCL2)
RN  - 0 (Herpes Simplex Virus Vaccines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein B, Simplexvirus)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/immunology
MH  - Chemokine CCL2/*genetics/immunology
MH  - Female
MH  - Herpes Simplex/*immunology/virology
MH  - Herpes Simplex Virus Vaccines/*immunology
MH  - Herpesvirus 1, Human/immunology
MH  - *Immunity, Mucosal
MH  - Immunization
MH  - Interferon-gamma/metabolism
MH  - Macrophage Inflammatory Proteins/*genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - *Plasmids/genetics
MH  - Th1 Cells/immunology
MH  - Vaccines, DNA
MH  - Vagina/immunology
MH  - Vaginal Diseases/immunology/prevention & control/virology
MH  - Viral Envelope Proteins/genetics
PMC - PMC113952
EDAT- 2001/01/03 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/01/01
CRDT- 2001/01/03 11:00
PHST- 2001/01/03 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/01/03 11:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 1386 [pii]
AID - 10.1128/JVI.75.2.569-578.2001 [doi]
PST - ppublish
SO  - J Virol. 2001 Jan;75(2):569-78. doi: 10.1128/JVI.75.2.569-578.2001.