PMID- 11134342
OWN - NLM
STAT- MEDLINE
DCOM- 20010202
LR  - 20240104
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 21
IP  - 2
DP  - 2001 Jan
TI  - The RNase P associated with HeLa cell mitochondria contains an essential RNA 
      component identical in sequence to that of the nuclear RNase P.
PG  - 548-61
AB  - The mitochondrion-associated RNase P activity (mtRNase P) was extensively 
      purified from HeLa cells and shown to reside in particles with a sedimentation 
      constant ( approximately 17S) very similar to that of the nuclear enzyme (nuRNase 
      P). Furthermore, mtRNase P, like nuRNase P, was found to process a mitochondrial 
      tRNA(Ser(UCN)) precursor [ptRNA(Ser(UCN))] at the correct site. Treatment with 
      micrococcal nuclease of highly purified mtRNase P confirmed earlier observations 
      indicating the presence of an essential RNA component. Furthermore, 
      electrophoretic analysis of 3'-end-labeled nucleic acids extracted from the peak 
      of glycerol gradient-fractionated mtRNase P revealed the presence of a 
      340-nucleotide RNA component, and the full-length cDNA of this RNA was found to 
      be identical in sequence to the H1 RNA of nuRNase P. The proportions of the 
      cellular H1 RNA recovered in the mitochondrial fractions from HeLa cells purified 
      by different treatments were quantified by Northern blots, corrected on the basis 
      of the yield in the same fractions of four mitochondrial nucleic acid markers, 
      and shown to be 2 orders of magnitude higher than the proportions of 
      contaminating nuclear U2 and U3 RNAs. In particular, these experiments revealed 
      that a small fraction of the cell H1 RNA (of the order of 0.1 to 0.5%), 
      calculated to correspond to approximately 33 to approximately 175 intact 
      molecules per cell, is intrinsically associated with mitochondria and can be 
      removed only by treatments which destroy the integrity of the organelles. In the 
      same experiments, the use of a probe specific for the RNA component of RNase MRP 
      showed the presence in mitochondria of 6 to 15 molecules of this RNA per cell. 
      The available evidence indicates that the levels of mtRNase P detected in HeLa 
      cells should be fully adequate to satisfy the mitochondrial tRNA synthesis 
      requirements of these cells.
FAU - Puranam, R S
AU  - Puranam RS
AD  - Division of Biology, California Institute of Technology, Pasadena, California 
      91125, USA.
FAU - Attardi, G
AU  - Attardi G
LA  - eng
GR  - R01 GM011726/GM/NIGMS NIH HHS/United States
GR  - GM11726/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Biomarkers)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Catalytic)
RN  - 0 (RNA, Mitochondrial)
RN  - 0 (RNA, Nuclear)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (RNA, Small Nucleolar)
RN  - 0 (RNA, U3 small nucleolar)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (U2 small nuclear RNA)
RN  - 63231-63-0 (RNA)
RN  - 9014-25-9 (RNA, Transfer)
RN  - EC 3.1.- (Endoribonucleases)
RN  - EC 3.1.26.5 (RPP14 protein, human)
RN  - EC 3.1.26.5 (Ribonuclease P)
RN  - EC 3.1.26.5 (ribonuclease P, E coli)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
RN  - KOO5CM684H (Digitonin)
SB  - IM
CIN - Mol Cell Biol. 2001 Dec;21(23):8236-7. PMID: 11710332
MH  - Biomarkers/analysis
MH  - Catalysis
MH  - Cell Nucleus/*enzymology/genetics
MH  - Cloning, Molecular
MH  - Digitonin/metabolism
MH  - Endoribonucleases/chemistry/*genetics/isolation & purification/metabolism
MH  - Escherichia coli/genetics
MH  - *Escherichia coli Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Micrococcal Nuclease/metabolism
MH  - Mitochondria/*enzymology/genetics
MH  - RNA/analysis/*genetics
MH  - RNA Precursors/genetics/metabolism
MH  - RNA, Catalytic/chemistry/*genetics/isolation & purification/metabolism
MH  - RNA, Mitochondrial
MH  - RNA, Nuclear/analysis/*genetics
MH  - RNA, Small Nuclear/analysis
MH  - RNA, Small Nucleolar/analysis
MH  - RNA, Transfer/genetics/metabolism
MH  - Ribonuclease P
MH  - Ribonucleoproteins/chemistry/genetics/isolation & purification/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Analysis, DNA
PMC - PMC86618
EDAT- 2001/01/03 11:00
MHDA- 2001/03/03 10:01
PMCR- 2001/01/01
CRDT- 2001/01/03 11:00
PHST- 2001/01/03 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2001/01/03 11:00 [entrez]
PHST- 2001/01/01 00:00 [pmc-release]
AID - 1194 [pii]
AID - 10.1128/MCB.21.2.548-561.2001 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2001 Jan;21(2):548-61. doi: 10.1128/MCB.21.2.548-561.2001.