PMID- 11134589
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 887
IP  - 1
DP  - 2000 Dec 22
TI  - Nerve growth factor applied onto the olfactory epithelium alleviates degenerative 
      changes of the olfactory receptor neurons following axotomy.
PG  - 53-62
AB  - The olfactory neuroepithelium of the mammalian nervous system manifests 
      continuous neurogenesis throughout life. Recent studies suggest that neurotrophic 
      factors and their receptors may play a role in the regulation of development and 
      regeneration in the olfactory system. However, there have been very few in vivo 
      studies investigating the effect of exogenous neurotrophic factors in the 
      olfactory system. In the present study, nerve growth factor (NGF) and 
      brain-derived neurotrophic factor (BDNF) were administered into the rat olfactory 
      mucosa for 5 days just after the transection of the olfactory nerve. We then 
      examined the effect of exogenous neurotrophic factors on the degenerative changes 
      in axotomized olfactory receptor neurons (ORNs). Further, we examined the 
      location of their receptors, Trk A and Trk B. We found that both mature and 
      immature ORNs expressed more intense signals for olfactory marker protein and 
      beta-tubulin mRNAs, respectively, when NGF was applied to the axotomized 
      olfactory neuroepithelium for 5 days, compared to the ORNs of saline-treated 
      controls. BDNF at a 10 microg total dose did not show this effect. The effect of 
      NGF applied onto the olfactory epithelium is consistent with the 
      immunohistochemical finding that Trk A was present in the dendrites and axon 
      bundles in normal and axotomized ORNs. These results suggest that NGF may protect 
      the degenerative changes in mature and immature ORNs following axotomy through 
      the binding to the Trk A receptor located on the surface of the olfactory 
      epithelium.
FAU - Yasuno, H
AU  - Yasuno H
AD  - Department of Anatomy and Neuroscience, Hyogo College of Medicine, 663-8501, 
      Hyogo, Japan.
FAU - Fukazawa, K
AU  - Fukazawa K
FAU - Fukuoka, T
AU  - Fukuoka T
FAU - Kondo, E
AU  - Kondo E
FAU - Sakagami, M
AU  - Sakagami M
FAU - Noguchi, K
AU  - Noguchi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Olfactory Marker Protein)
RN  - 0 (Omp protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/*pharmacology/therapeutic use
MH  - Male
MH  - Nerve Degeneration/drug therapy/*metabolism
MH  - Nerve Growth Factor/*pharmacology/therapeutic use
MH  - Nerve Tissue Proteins/*drug effects/metabolism
MH  - Olfactory Marker Protein
MH  - Olfactory Mucosa/*drug effects/metabolism
MH  - Olfactory Receptor Neurons/*drug effects/metabolism
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkA/metabolism
MH  - Receptor, trkB/metabolism
EDAT- 2001/01/03 11:00
MHDA- 2001/03/07 10:01
CRDT- 2001/01/03 11:00
PHST- 2001/01/03 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2001/01/03 11:00 [entrez]
AID - S0006-8993(00)02966-8 [pii]
AID - 10.1016/s0006-8993(00)02966-8 [doi]
PST - ppublish
SO  - Brain Res. 2000 Dec 22;887(1):53-62. doi: 10.1016/s0006-8993(00)02966-8.