PMID- 11135292
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20191104
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 42
IP  - 6
DP  - 2000 Dec 15
TI  - Actin polymerization in response to different chemoattractants is reduced in 
      granulocytes from chronic myeloid leukemia patients.
PG  - 379-86
AB  - Chronic myeloid leukemia (CML), a hematopoietic stem cell disorder, is 
      characterized by the presence of Philadelphia chromosome (Ph1). Earlier studies 
      have shown that various functions, such as chemotaxis, fluid phase pinocytosis, 
      phagocytosis, and degranulation in response to chemotactic peptide 
      formyl-methionyl-leucyl-phenylalanine (fMLP), were defective in polymorphonuclear 
      leukocytes (PMNL) from CML patients. These functions depend on actin 
      microfilaments (MF). Further studies showed that fMLP-induced actin 
      polymerization was lower in CML PMNL. To see if this defect is specific to 
      stimulation by fMLP alone or is a global phenomenon involving other 
      chemoattractant receptors, chemotaxis and actin polymerization were studied in 
      response to fMLP, an analog of fMLP, formyl-methionine-1 aminocyclooctane 1 
      carboxylic acid-phenyalanine-O-methionine (FACC8), platelet-activating factor 
      (PAF), and leukotriene B4 (LTB4). These compounds bind to different 
      chemoattractant receptors. Chemotaxis and actin polymerization in response to all 
      four chemoattractants were significantly lower in CML PMNL compared with PMNL 
      from normal subjects and were differentially affected for the different 
      chemoattractants. These results suggest a global phenomenon involving all four 
      chemoattractant-stimulated pathways. This lower amount of F-actin may be 
      responsible for the defective chemotaxis seen in these cells.
FAU - Radhika, V
AU  - Radhika V
AD  - Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai, India.
FAU - Naik, N R
AU  - Naik NR
FAU - Advani, S H
AU  - Advani SH
FAU - Bhisey, A N
AU  - Bhisey AN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Actins)
RN  - 0 (Chemotactic Factors)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Actins/*metabolism
MH  - Chemotactic Factors/*pharmacology
MH  - *Chemotaxis
MH  - Flow Cytometry
MH  - Granulocytes/drug effects/*metabolism
MH  - Humans
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*metabolism
MH  - Microscopy, Fluorescence
MH  - N-Formylmethionine Leucyl-Phenylalanine/agonists/pharmacology
EDAT- 2001/01/03 11:00
MHDA- 2001/03/03 10:01
CRDT- 2001/01/03 11:00
PHST- 2001/01/03 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2001/01/03 11:00 [entrez]
AID - 10.1002/1097-0320(20001215)42:6<379::AID-CYTO1005>3.0.CO;2-Q [pii]
AID - 10.1002/1097-0320(20001215)42:6<379::aid-cyto1005>3.0.co;2-q [doi]
PST - ppublish
SO  - Cytometry. 2000 Dec 15;42(6):379-86. doi: 
      10.1002/1097-0320(20001215)42:6<379::aid-cyto1005>3.0.co;2-q.